与人类DNA中一个新的保守短分散重复元件家族成员相关的结构和转录特性。

Structural and transcriptional properties associated with a member of a new family of conserved short dispersed repeated elements in human DNA.

作者信息

Humphries P, MacCabe A P, Spencer R A, Humphries M M, Pearson C

出版信息

Gene. 1985;39(2-3):255-61. doi: 10.1016/0378-1119(85)90320-8.

Abstract

The sequence of a 500-bp segment of human DNA containing a member of the 'A3' family of dispersed repeated elements, a family hitherto uncharacterized in human or rodent DNAs, is presented. The repeated element maps to a region of 270 bp, within which the most notable features are 17-mer direct repeats separated by 190 bp. A probe containing the repeated element, together with flanking single-copy DNA illuminates a series of multiple bands in blot transfers of restriction digests of human, mouse and hamster DNAs. However, the flanking single-copy DNA hybridizes to human, but not to rodent DNA. The same probe illuminates homologous transcripts occurring in a single size class of 0.8 kb in total RNA from a variety of human and mouse cells, levels of which vary approx. 100-fold in the tissues analyzed. Poly(A)+ RNAs from EJ-transformed 3T3 cells and mouse fibrosarcomas have been analysed and found also to contain an homologous 0.8-kb transcript.

摘要

本文给出了一段500碱基对（bp）的人类DNA序列，其中包含一个属于“A3”家族的分散重复元件，该家族在人类或啮齿动物DNA中尚未被鉴定。这个重复元件定位在一个270 bp的区域内，其中最显著的特征是间隔190 bp的17聚体直接重复序列。一个包含该重复元件以及侧翼单拷贝DNA的探针，在人类、小鼠和仓鼠DNA的限制性酶切片段印迹转移中显示出一系列多条带。然而，侧翼单拷贝DNA与人类DNA杂交，但不与啮齿动物DNA杂交。相同的探针在来自多种人类和小鼠细胞的总RNA中，显示出一个单一大小类别的0.8 kb同源转录本，在所分析的组织中其水平大约相差100倍。对EJ转化的3T3细胞和小鼠纤维肉瘤的聚腺苷酸加尾（Poly(A)+）RNA进行了分析，发现也含有一个同源的0.8 kb转录本。