Byrd Doratha A, Gomez Maria F, Hogue Stephanie R, Burns Jessica R, Smith Nate, Sampson Joshua, Loftfield Erikka, Wolf Patricia G, Wan Yunhu, Warner Andrew, Hicks Belynda, Dagnall Casey, Jones Kristine, Kim Youngchul, Xu Jin, Shi Jianxin, Sinha Rashmi, Vogtmann Emily
Department of Cancer Epidemiology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida, USA.
Department of Gastrointestinal Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida, USA.
Cancer Med. 2025 Sep;14(18):e71048. doi: 10.1002/cam4.71048.
The gut microbiome and bile acids (BAs) likely influence colorectal cancer (CRC) development and disparities. We conducted a nested case-control study of the associations of the colon tissue microbiome and circulating BAs with colorectal adenoma prevalence in the previously conducted multi-center Colorectal Neoplasia Screening with Colonoscopy in Average-Risk Women Regional Navy/Army Medical Centers study (CONCeRN).
We individually matched 143 women with adenoma to 279 without adenoma. Using 16S rRNA gene sequencing, we assessed alpha and beta diversity, taxonomic abundance, and co-abundance groups (CAGs). Fasting serum was analyzed for 13 primary and secondary BAs.
The presence of oral-originating Porphyromonas was positively associated with adenomas (odds ratio [OR] and 95% confidence interval [CI] = 2.50 [1.18, 5.30]; p = 0.02). Race and study center explained statistically significant percentages of variation in the beta diversity matrices. BAs were generally positively associated with adenomas, though these results were not statistically significant.
Overall, our findings suggest the colon tissue microbiome may differ by race and geography, and that certain oral-originating bacteria may be positively associated with adenomas.
肠道微生物群和胆汁酸（BAs）可能影响结直肠癌（CRC）的发生及差异。在先前开展的多中心“海军/陆军地区医疗中心平均风险女性结肠镜检查结直肠肿瘤筛查研究（CONCeRN）”中，我们进行了一项巢式病例对照研究，以探讨结肠组织微生物群和循环胆汁酸与结直肠腺瘤患病率之间的关联。
我们将143例患有腺瘤的女性与279例无腺瘤的女性进行个体匹配。使用16S rRNA基因测序，我们评估了α和β多样性、分类丰度和共丰度组（CAGs）。对空腹血清进行13种初级和次级胆汁酸分析。
口腔来源的卟啉单胞菌的存在与腺瘤呈正相关（比值比[OR]和95%置信区间[CI]=2.50[1.18，5.30]；p=0.02）。种族和研究中心在β多样性矩阵的变异中所占百分比具有统计学意义。胆汁酸一般与腺瘤呈正相关，尽管这些结果无统计学意义。
总体而言，我们的研究结果表明，结肠组织微生物群可能因种族和地理位置而异，某些口腔来源的细菌可能与腺瘤呈正相关。
