School of Mechanical Engineering, Yangzhou University, Yangzhou, 225127, China.
School of Mechanical Engineering, Yangzhou University, Yangzhou, 225127, China.
Biosens Bioelectron. 2024 Aug 1;257:116338. doi: 10.1016/j.bios.2024.116338. Epub 2024 Apr 25.
Foodborne pathogens have a substantial bearing on food safety and environmental health. The development of automated, portable and compact devices is essential for the on-site and rapid point-of-care testing (POCT) of bacteria. Here, this work developed a micro-automated microfluidic device for detecting bacteria, such as Escherichia coli (E. coli) O157:H7, using a seashell-like microfluidic chip (SMC) as an analysis and mixing platform. The automated device integrates a colorimetric/fluorescent system for the metabolism of copper (Cu) by E. coli affecting o-phenylenediamine (OPD) for concentration analysis. A smartphone was used to read the RGB data of the chip reaction reservoir to detect colorimetric and fluorescence patterns in the concentration range of 10-10 CFU mL. The automated device overcomes the low efficiency and tedious steps of traditional detection and enables high-precision automated detection that can be applied to POCT in the field, providing an ideal solution for broadening the application of E. coli detection.
食源性致病菌对食品安全和环境卫生有重大影响。开发自动化、便携式和紧凑型设备对于现场和快速即时检测（POCT）细菌至关重要。本研究开发了一种微型自动化微流控装置，使用贝壳状微流控芯片（SMC）作为分析和混合平台来检测细菌，如大肠杆菌（E. coli）O157：H7。该自动化设备集成了比色/荧光系统，用于大肠杆菌代谢铜（Cu）对邻苯二胺（OPD）的影响，用于浓度分析。智能手机用于读取芯片反应池的 RGB 数据，以检测浓度范围内 10-10 CFU mL 的比色和荧光模式。自动化设备克服了传统检测效率低和步骤繁琐的问题，实现了高精度的自动化检测，可应用于现场 POCT，为扩大大肠杆菌检测的应用提供了理想的解决方案。
