Luo Wei, He Xiaona, Chen Yuxue, Xu Yewen, Zhuang Yongliang, Ding Yangyue, Fan Xuejing
Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China.
Yunnan Technology Innovation Center of Woody Oil, Kunming 650201, China.
Foods. 2025 Sep 5;14(17):3112. doi: 10.3390/foods14173112.
() is renowned for its tolerance to gastric acid and adaptability to bile and alkaline conditions, and is crucial for intestinal health and immune regulation. In this study, integrated transcriptomic and proteomic analyses were employed to elucidate the response mechanisms of under osmotic stress, induced by exposure to 0.6 M sodium lactate, which elevates environmental osmotic pressure. It was shown that 792 differentially expressed genes and 138 differentially expressed proteins were detected in ATCC 53103 treated with osmotic stress. The differential regulation of these genes/proteins mainly includes the inhibition of fatty acid metabolism with membrane structural remodeling (downregulation of the acetyl coenzyme A carboxylase family and fatty acid binding protein family expression), dynamic homeostasis of amino acid metabolism (restriction of the synthesis of histidine, cysteine, leucine, etc., and enhancement of the catabolism of lysine, tryptophan, etc.), and survival-oriented reconfiguration of carbohydrate metabolism (gene expression related to the glycolytic pathway increases, while gene expression related to the pentose phosphate pathway decreases). These synergistic alterations in metabolic regulation may facilitate the adaptive response of ATCC 53103 to osmotic stress. Overall, our findings deepen the current understanding of the stress response mechanisms in lactic acid bacteria and offer novel insights into the survival strategies employed by ATCC 53103 under hyperosmotic conditions.
（）以其对胃酸的耐受性以及对胆汁和碱性条件的适应性而闻名，对肠道健康和免疫调节至关重要。在本研究中，采用整合转录组学和蛋白质组学分析来阐明（）在暴露于0.6 M乳酸钠导致环境渗透压升高所引发的渗透胁迫下的应答机制。结果表明，在经受渗透胁迫处理的（）ATCC 53103中检测到792个差异表达基因和138个差异表达蛋白质。这些基因/蛋白质的差异调节主要包括脂肪酸代谢受抑制并伴有膜结构重塑（乙酰辅酶A羧化酶家族和脂肪酸结合蛋白家族表达下调）、氨基酸代谢的动态稳态（组氨酸、半胱氨酸、亮氨酸等的合成受限，而赖氨酸、色氨酸等的分解代谢增强）以及以生存为导向的碳水化合物代谢重配置（糖酵解途径相关基因表达增加，而磷酸戊糖途径相关基因表达减少）。这些代谢调节中的协同改变可能有助于（）ATCC 53103对渗透胁迫的适应性应答。总体而言，我们的研究结果加深了当前对乳酸菌应激反应机制的理解，并为ATCC 53103在高渗条件下所采用的生存策略提供了新的见解。
