Sun Yue, Zhao Yiduo, Jia Minshi, Zhang Xudong, Zhou Xixuan, Li Shengnan, Wu Zedong, Pi Zhi
Academy of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, China.
Key Laboratory of Sugar Beet Genetic Breeding, Heilongjiang University, Harbin 150080, China.
Plants (Basel). 2025 Sep 2;14(17):2747. doi: 10.3390/plants14172747.
Sugar beet ( L.), a biennial sugar crop, provides about 16% of the world's sucrose production. PEG and -mediated transformation have been established for sugar beet. However, the traditional transformation of sugar beet is time-consuming, low efficiency, and dependent on tissue regeneration. Recently, the use of for genetic transformation without tissue culture has become a new possibility. Here, we describe an optimized -mediated transformation for the generation of composite sugar beet without tissue culture. By dipping K599 colonies onto a wound of hypocotyl and petiole, about 81.7% and 51.1% of shoots and leaves could be induced to produce adventitious roots. Of these, more than 60% of the explants contained transformed adventitious roots. Specifically, we discovered that the transformation efficiency was significantly improved when the promoter was employed instead of the promoter. The transformation in adventitious roots was also validated by qRT-PCR and Western blot at the transcriptional and translational levels. The transformed adventitious roots have great potential for the study of taproot development, sugar accumulation, and resistance to root diseases, which is closely related to sugar beet yield and quality.
甜菜（Beta vulgaris L.）是一种二年生糖料作物，提供了全球约16%的蔗糖产量。聚乙二醇（PEG）介导的转化方法已在甜菜中建立。然而，传统的甜菜转化方法耗时、效率低且依赖于组织再生。最近，利用无需组织培养的方法进行基因转化成为了一种新的可能性。在此，我们描述了一种优化的无需组织培养生成复合甜菜的农杆菌介导转化方法。通过将农杆菌K599菌落蘸到下胚轴和叶柄的伤口上，约81.7%的芽和51.1%的叶可被诱导产生不定根。其中，超过60%的外植体含有转化的不定根。具体而言，我们发现使用CaMV 35S启动子而非35S promoter时转化效率显著提高。不定根中的转化也通过qRT-PCR和蛋白质免疫印迹在转录和翻译水平上得到了验证。转化的不定根在主根发育、糖分积累和根病抗性研究方面具有巨大潜力，而这些与甜菜的产量和品质密切相关。
