Pizano-Andrade Juan Carlos, Vargas-Guerrero Belinda, Vargas-Radillo Jesus, Domínguez-Rosales José Alfredo, Montalvo-González Efigenia, Rodriguez-Macias Ramon, Garcia-López Pedro Macedonio, Del Rocio Romero-Verdín Margarita, Ruiz López Mario Alberto
Botany and Zoology Department, CUCBA, University of Guadalajara, Zapopan 44600, Jalisco, Mexico.
Department of Molecular Biology and Genomics, University Center for Health Sciences, University of Guadalajara, Guadalajara 44340, Jalisco, Mexico.
Molecules. 2025 Aug 28;30(17):3518. doi: 10.3390/molecules30173518.
A current problem is the increase in skin damage, including cancer, caused mainly by prolonged exposure to ultraviolet rays from sunlight. Therefore, the aim of this work was to study the photoprotective effect to ultraviolet radiation of phenolics and acetogenic-rich extracts obtained from leaves applied to the skin of rats by means of gene expression in and , involved in tumor processes due to cell damage, in addition to the content of phenols, acetogenins and antioxidant activity present in the extract, which presented a total phenol content of 61.5 mg EAG/100 g of dry sample and flavonoids of 50 mg EQ/100 g. HPLC analysis revealed that the major compound was shikimic acid, followed by gallocatechin and 13 other phenols. DPPH analysis showed an inhibition of 64.37% and FRAP showed a value of 28,880 µmol Eq trolox/mL. The presence of acetogenins was verified by Kedde's reagent in HPTLC. Histopathological findings in the treated groups (T4, T5) suggest thickening of the epidermis, which could be due to fibroblast proliferation. The results show a higher increase in and gene expression with the tested extracts compared to the positive control group, so it can be concluded that the extracts have positive effects.
当前存在的一个问题是皮肤损伤增加，包括癌症，主要是由于长期暴露于阳光中的紫外线所致。因此，本研究的目的是通过参与细胞损伤所致肿瘤过程的 和 中的基因表达，研究从树叶中提取的富含酚类和产乙酸成分的提取物对大鼠皮肤紫外线辐射的光保护作用，此外还研究了提取物中酚类、产乙酸成分和抗氧化活性的含量，该提取物的总酚含量为61.5 mg EAG/100 g干样品，黄酮类化合物含量为50 mg EQ/100 g。高效液相色谱分析表明，主要化合物是莽草酸，其次是没食子儿茶素和其他13种酚类。二苯基苦味酰基自由基分析显示抑制率为64.37%，铁离子还原抗氧化能力分析显示值为28,880 µmol Eq Trolox/mL。通过高效薄层色谱中的凯氏试剂验证了产乙酸成分的存在。治疗组（T4、T5）的组织病理学结果表明表皮增厚，这可能是由于成纤维细胞增殖所致。结果显示，与阳性对照组相比，测试提取物使 和 基因表达有更高的增加，因此可以得出结论，提取物具有积极作用。
