Fu Yuna, Wang Jianhua, Gu Di, Zhang Letian
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
Molecules. 2025 Aug 30;30(17):3558. doi: 10.3390/molecules30173558.
Electric fields are emerging as powerful tools to actively regulate biomolecular interactions at biointerfaces. In this study, we investigated how varying electric field strengths (0-100 mV/mm) influence the interfacial interaction between human serum albumin (HSA) and six tyrosine kinase inhibitors (TKIs): imatinib, bosutinib, dasatinib, nilotinib, ponatinib, and radotinib. Using atomic force microscopy (AFM), we quantified changes in adhesion force, specific () and non-specific () force, friction behavior, and protein morphology. Increasing field strength led to significant reductions in adhesion force (22-47%), (27-44%), (38-53%), friction force (38-67%) and constant-load friction force (43-54%), along with decreased protein average surface height and roughness, indicating electric field-induced molecular compaction and interface smoothing. Notably, more hydrophobic TKIs showed greater responsiveness. These findings highlight the potential of electric fields to modulate protein-drug interactions in a controllable manner, offering a new strategy for the development of electrically tunable drug delivery systems and smart biomedical interfaces.
电场正成为在生物界面积极调节生物分子相互作用的有力工具。在本研究中，我们研究了不同电场强度（0 - 100 mV/mm）如何影响人血清白蛋白（HSA）与六种酪氨酸激酶抑制剂（TKIs）：伊马替尼、博舒替尼、达沙替尼、尼洛替尼、波纳替尼和拉多替尼之间的界面相互作用。使用原子力显微镜（AFM），我们量化了粘附力、特异性（）和非特异性（）力、摩擦行为以及蛋白质形态的变化。电场强度增加导致粘附力（22 - 47%）、（27 - 44%）、（38 - 53%）、摩擦力（38 - 67%）和恒定负载摩擦力（43 - 54%）显著降低，同时蛋白质平均表面高度和粗糙度减小，表明电场诱导分子压实和界面平滑。值得注意的是，疏水性更强的TKIs表现出更大的响应性。这些发现突出了电场以可控方式调节蛋白质 - 药物相互作用的潜力，为开发电可调药物递送系统和智能生物医学界面提供了新策略。
