Tang Shuai, Wang Jiajia, Yan Kuo, Yu Zhixin, Li Jitao
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao 266237, China.
Int J Mol Sci. 2025 Aug 28;26(17):8339. doi: 10.3390/ijms26178339.
Solute carrier family 12 () encodes electroneutral cation-coupled chloride cotransporters responsible for transmembrane ion transport (Na, K, and Cl), which play a critical role in aquatic osmoregulation. However, the gene of () has not been systematically identified or functionally characterized. In this study, six genes were identified across the genome and classified into N(K)CC, KCC, CCC9, and CIP subfamilies. Three homologous genes (, , and ) were reported for the first time in crustaceans. The family exhibited distinct expression patterns in response to low-salinity, high-alkalinity, and saline-alkaline stress. was highly expressed in the gill, and its expression was closely correlated with saline-alkaline acclimation. Additionally, knockdown decreased survival under saline-alkaline stress. Thus, plays critical roles in osmotic regulation and saline-alkaline acclimation. This study provides crucial insights into 's saline-alkaline tolerance mechanisms, and the discovery of multiple homologs fills a gap in the crustacean gene family research.
溶质载体家族12（）编码电中性阳离子偶联氯化物共转运体，负责跨膜离子运输（钠、钾和氯），这在水生生物渗透调节中起关键作用。然而，（）的基因尚未得到系统鉴定或功能表征。在本研究中，在基因组中鉴定出六个基因，并将其分为N（K）CC、KCC、CCC9和CIP亚家族。首次在甲壳类动物中报道了三个同源基因（、和）。该家族在低盐度、高碱度和盐碱胁迫下表现出不同的表达模式。在鳃中高表达，其表达与盐碱适应密切相关。此外，敲低在盐碱胁迫下降低了的存活率。因此，在渗透调节和盐碱适应中起关键作用。本研究为的盐碱耐受机制提供了重要见解，多个同源物的发现填补了甲壳类动物基因家族研究的空白。
