Yang Jiao, Li Shuhan, Li Xuan, Wang Tiantian, Deng Huihua, Jia Yuanyuan, Meng Dianhuai
Rehabilitation Center, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.
School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
Front Neurol. 2025 Aug 28;16:1595429. doi: 10.3389/fneur.2025.1595429. eCollection 2025.
There have been many studies on the relationship between sex hormones and stress, mood, blood pressure, etc., but its impact on the incidence of stroke remains unknown.
To investigate the expression levels of hypothalamic-pituitary-gonadal (HPG) axis related hormones such as testosterone (T) and progesterone (P) in hair before stroke and their effects on the risk of stroke.
48 patients with stroke were recruited from November 2022 to May 2023 as the observation group and 35 healthy subjects were recruited as the control group. There was no obvious difference in age, gender and BMI between the two groups ( > 0.05). T and P levels in hair were tested by LC-MS/MS, and the correlation with the risk of stroke was analyzed.
The T and P levels of hair before the onset of stroke in the observation group were significantly lower than those in the control group ( < 0.01). The T level of men's hair before the onset of stroke in the observation group was significantly lower than those in the control group ( < 0.05). The T and P levels of women's hair before the onset of stroke in the observation group were significantly lower than those in the control group ( < 0.01). The results of ROC curve showed that the cut-off value of T level in men's hair before the onset of stroke was 4.35 pg/mg, the AUC was 0.690 (0.545, 0.835), the sensitivity was 62.50%, and the specificity was 82.61%. The cut-off value of T level in women's hair before the onset of stroke was 5.00 pg/mg, the AUC was 0.818 (0.658, 0.978), the sensitivity was 75.00%, and the specificity was 83.33%. The cut-off value of P level in women's hair before the onset of stroke was 8.00 pg/mg, the AUC was 0.891 (0.754, 1.000), the sensitivity was 81.25%, and the specificity was 100.00%.
This preliminary report is the first to suggest that HPG axis hormones such as T and P in hair could have predictive value in screening for stroke risk.
关于性激素与压力、情绪、血压等之间的关系已有许多研究，但其对中风发病率的影响仍不清楚。
探讨中风前头发中下丘脑-垂体-性腺（HPG）轴相关激素如睾酮（T）和孕酮（P）的表达水平及其对中风风险的影响。
选取2022年11月至2023年5月的48例中风患者作为观察组，选取35例健康受试者作为对照组。两组在年龄、性别和BMI方面无明显差异（>0.05）。采用液相色谱-串联质谱法检测头发中的T和P水平，并分析其与中风风险的相关性。
观察组中风发病前头发中的T和P水平显著低于对照组（<0.01）。观察组男性中风发病前头发中的T水平显著低于对照组（<0.05）。观察组女性中风发病前头发中的T和P水平显著低于对照组（<0.01）。ROC曲线结果显示，男性中风发病前头发中T水平的截断值为4.35 pg/mg，AUC为0.690（0.545，0.835），灵敏度为62.50%，特异性为82.61%。女性中风发病前头发中T水平的截断值为5.00 pg/mg，AUC为0.818（0.658，0.978），灵敏度为75.00%，特异性为83.33%。女性中风发病前头发中P水平的截断值为8.00 pg/mg，AUC为0.891（0.754，1.000），灵敏度为81.25%，特异性为100.00%。
本初步报告首次表明，头发中的T和P等HPG轴激素在中风风险筛查中可能具有预测价值。
