Demir Irem, Seker Gamze, Aysan Arzu, Dandan Doganci Merve, Davarcı Derya, Doganci Erdinc
Department of Chemistry, Gebze Technical University, 41400 Gebze, Kocaeli, Turkey.
Department of Molecular Biology and Genetics, Gebze Technical University, 41400 Gebze, Kocaeli, Turkey.
ACS Omega. 2025 Aug 28;10(35):40567-40578. doi: 10.1021/acsomega.5c06436. eCollection 2025 Sep 9.
In this study, antimicrobial poly-(lactic acid)/poly-(ethylene glycol) (PLA/PEG) biofilms containing imidazole derivative cyclotriphosphazene compounds were prepared by using the solvent casting method. The mechanical, thermal, and biologically active properties of the obtained films were investigated. Biofilms were formed by adding the synthesized fully substituted methylimidazole and benzimidazole cyclotriphosphazene compounds (MCp and BCp) to the PLA/PEG mixture as additives at different rates. Tensile tests were performed on these films to examine their mechanical properties, including Young's modulus, elongation at break, and yield strength. It was observed that the mechanical properties of the films were slightly negatively affected by the addition of additives to the PLA/PEG films. When thermal stability analyses were examined, it was determined that the addition of the additives increased slightly, although not significantly. Antimicrobial tests were performed using Gram-negative ATCC 53323 and Gram-positive ATCC 29213 bacterial strains for MCp and BCp and PLA/PEG composite films MCp and BCp, and PLA/PEG composite films containing these compounds. It was observed that MCp was much more effective than BCp in inhibiting the growth of Gram-positive and Gram-negative bacteria. Additionally, the PLA/PEG/MCp and PLA/PEG/BCp films had antibiofilm activity.
在本研究中，采用溶剂浇铸法制备了含有咪唑衍生物环三磷腈化合物的抗菌聚乳酸/聚乙二醇（PLA/PEG）生物膜。对所得薄膜的机械性能、热性能和生物活性进行了研究。通过以不同比例向PLA/PEG混合物中添加合成的全取代甲基咪唑和苯并咪唑环三磷腈化合物（MCp和BCp）作为添加剂来形成生物膜。对这些薄膜进行拉伸试验以检测其机械性能，包括杨氏模量、断裂伸长率和屈服强度。观察到向PLA/PEG薄膜中添加添加剂会对薄膜的机械性能产生轻微负面影响。在检查热稳定性分析时，确定添加剂的添加虽未显著增加，但略有增加。使用革兰氏阴性ATCC 53323和革兰氏阳性ATCC 29213细菌菌株对MCp和BCp以及PLA/PEG复合薄膜MCp和BCp以及含有这些化合物的PLA/PEG复合薄膜进行抗菌试验。观察到MCp在抑制革兰氏阳性和革兰氏阴性细菌生长方面比BCp有效得多。此外，PLA/PEG/MCp和PLA/PEG/BCp薄膜具有抗生物膜活性。
