Franco-Gedda Lana Pacheco, Rodrigues Karina, Noll Matias, Mendes Marcela Moraes, Horst Maria Aderuza
Nutritional Genomics Research Group, Faculty of Nutrition Universidade Federal de Goiás (UFG) Goiânia Goiás Brazil.
Instituto Federal Goiano (IF Goiano) Ceres Goiás Brazil.
Health Sci Rep. 2025 Sep 11;8(9):e70948. doi: 10.1002/hsr2.70948. eCollection 2025 Sep.
Vitamin D deficiency is a major public health issue, with varying individual responses to supplementation. Genetic factors, especially single-nucleotide polymorphisms (SNPs) in Vitamin D metabolism genes, likely play a key role. This protocol proposes a systematic review to explore how genetic variability affects serum 25-hydroxyvitamin D [25(OH)D] levels after supplementation.
This protocol adheres to the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P). The literature search will be conducted across MEDLINE, Scopus, Web of Science, and Embase, without restrictions on publication date or language. The study selection will be guided by Population, Exposure, Comparator, Outcomes, Study Design (PECOS) framework, focusing on randomized clinical trials that report pre- and post-supplementation serum 25(OH)D levels alongside genotype data. Inclusion criteria comprise adults and elderly individuals, from both sexes and any ethnicity, who received Vitamin D supplementation and have SNPs data, while exclusion criteria reject studies with confounding factors such as pre-existing conditions or use of medications affecting Vitamin D status. Data extraction will include study characteristics, participant demographics, intervention details, SNPs, and serum 25(OH)D data. Inter-rater reliability will be assessed using Cohen's kappa coefficient. A descriptive synthesis will summarize the findings, and if feasible, a meta-analysis will be conducted. The primary outcome will be changes in serum 25(OH)D concentrations. Heterogeneity among studies will be quantified using the ² statistic. The methodological quality of studies will be assessed using the Joanna Briggs Institute checklist, and the overall certainty of evidence will be evaluated using the Grading of Recommendations, Assessment, Development, and Evaluations (GRADE) approach.
By identifying genetic subgroups with differential responses to vitamin D supplementation, the findings are expected to contribute to the development of personalized supplementation strategies. These insights may enhance health interventions by optimizing supplementation protocols based on genetic predispositions, ultimately improving health outcomes.
This protocol has been registered with International prospective register of systematic reviews (PROSPERO) ID: CRD42023449836.
维生素D缺乏是一个重大的公共卫生问题，个体对补充剂的反应各不相同。遗传因素，尤其是维生素D代谢基因中的单核苷酸多态性（SNP），可能起着关键作用。本方案提议进行一项系统评价，以探讨基因变异性如何影响补充后血清25-羟基维生素D[25(OH)D]水平。
本方案遵循系统评价与Meta分析方案的首选报告项目（PRISMA-P）。将在MEDLINE、Scopus、科学网和Embase上进行文献检索，不受出版日期或语言限制。研究选择将以人群、暴露、对照、结局、研究设计（PECOS）框架为指导，重点关注报告补充前后血清25(OH)D水平以及基因型数据的随机临床试验。纳入标准包括接受维生素D补充且有SNP数据的成年男女及老年个体，任何种族均可，而排除标准则排除存在如既有疾病或使用影响维生素D状态的药物等混杂因素的研究。数据提取将包括研究特征、参与者人口统计学、干预细节、SNP和血清25(OH)D数据。将使用Cohen's kappa系数评估评分者间信度。将进行描述性综合以总结研究结果，若可行，将进行Meta分析。主要结局将是血清25(OH)D浓度的变化。将使用I²统计量对研究间的异质性进行量化。将使用乔安娜·布里格斯研究所清单评估研究的方法学质量，并使用推荐分级、评估、制定与评价（GRADE）方法评估证据的总体确定性。
通过识别对维生素D补充有不同反应的基因亚组，预期研究结果将有助于制定个性化的补充策略。这些见解可能通过基于遗传易感性优化补充方案来加强健康干预，最终改善健康结局。
本方案已在国际系统评价前瞻性注册库（PROSPERO）注册，注册号：CRD42023449836。
