Barbosa Carolina Meneghin, Ortiz Mariangela Ivette Guanipa, Da Silva Julliana Andrade, Vieira-Junior Waldemir Francisco, Aguiar Flávio Henrique Baggio, Rischka Klaus, Lima Débora Alves Nunes Leite
Departamento de Odontologia Restauradora, Faculdade de Odontologia de Piracicaba (FOP), Universidade Estadual de Campinas (UNICAMP), P.O. BOX 52, Piracicaba, SP, 13414-903, Brazil.
Fraunhofer Institute for Manufacturing Technology and Advanced Materials IFAM, Bremen, Germany.
Odontology. 2025 Sep 16. doi: 10.1007/s10266-025-01200-7.
This in vitro study aimed to evaluate the changes in surface integrity of dental enamel after bleaching using gels with different compositions followed by an erosive challenge. Bovine enamel specimens (4 × 4 × 2 mm; n = 12/group) stored in artificial saliva were treated with bleaching gels containing carbamide peroxide (CP) or hydrogen peroxide (HP) with or without calcium polyphosphate (CaPP) followed by an erosive challenge. In Experiment 1, 10% commercial CP (CPC), CPC + 2% NaF, 10% manipulated CP (CPM), CPM + 2% NaF, 10% CP + 0.5 wt% CaPP, 10% CP + 1.5 wt% CaPP, manipulated gel without CP, or no bleaching were applied for 14 days. In Experiment 2, CP was switched with 35% HP, and all gels were applied for two sessions. Thereafter, all samples were subjected to a 5-day erosive challenge using 1% citric acid (pH 3.5) for 120 s. Enamel surface loss was assessed using contact profilometry and scanning electron microscopy (SEM). Statistical analysis was performed using a generalized linear model (α = 0.05). Compared with that in the CP + 0.5% CaPP and HP + 0.5% CaPP groups, enamel loss was significantly greater in the CPM and CPC groups and HPM and HPC groups in Experiments 1 and 2, respectively. SEM analysis revealed less surface degradation in the groups containing 0.5% CaPP, with greater preservation of the prismatic and interprismatic enamel structure. Both low- and high-concentration bleaching gels containing 0.5% CaPP improved enamel preservation after an erosive challenge compared to that with gels without CaPP. Thus, the addition of CaPP may help minimize enamel damage during bleaching by reducing mineral loss.
本体外研究旨在评估使用不同成分的凝胶进行漂白后,再经酸蚀挑战,牙釉质表面完整性的变化。将储存在人工唾液中的牛牙釉质标本(4×4×2毫米;每组n = 12)用含有过氧化脲(CP)或过氧化氢(HP)且添加或不添加聚磷酸钙(CaPP)的漂白凝胶处理,随后进行酸蚀挑战。在实验1中,应用10%市售CP(CPC)、CPC + 2% NaF、10%自制CP(CPM)、CPM + 2% NaF、10% CP + 0.5 wt% CaPP、10% CP + 1.5 wt% CaPP、不含CP的自制凝胶或不进行漂白处理,持续14天。在实验2中,将CP换成35% HP,所有凝胶均应用两个疗程。此后,所有样本使用1%柠檬酸(pH 3.5)进行为期5天的酸蚀挑战,持续120秒。使用接触轮廓仪和扫描电子显微镜(SEM)评估牙釉质表面损失。采用广义线性模型进行统计分析(α = 0.05)。与CP + 0.5% CaPP组和HP + 0.5% CaPP组相比,实验1和实验2中,CPM组和CPC组以及HPM组和HPC组的牙釉质损失分别显著更大。SEM分析显示,含0.5% CaPP的组表面降解较少,棱柱形和柱间质牙釉质结构保存得更好。与不含CaPP的凝胶相比,含低浓度和高浓度0.5% CaPP的漂白凝胶在酸蚀挑战后均能改善牙釉质的保存情况。因此,添加CaPP可能有助于通过减少矿物质流失来最小化漂白过程中对牙釉质的损伤。