Boyd Duangsamorn Boonwun, Mungmai Lapatrada, Vimolmangkang Sornkanok, Uputinan Sirada, Panprommin Dutrudi, Pathom-Aree Wasu, Auputinan Panitnart
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA.
Department of Cosmetic Sciences, School of Pharmaceutical Sciences, University of Phayao, Phayao, 56000, Thailand.
Bioresour Bioprocess. 2025 Sep 17;12(1):99. doi: 10.1186/s40643-025-00941-1.
The growing issue of antimicrobial resistance (AMR) in acne-related bacteria continues to undermine the effectiveness of standard treatments. This study examined the antibacterial potential of spp. leaf biomass. The leaves, which are often discarded or used locally as mulch to help retain soil moisture, are an underused agricultural byproduct. Leaf extracts were obtained from five cultivars using different ethanol and water-based solvent systems and were screened against ,, and . The 50% ethanolic extracts showed the strongest and most consistent inhibition patterns, with MIC values ranging from 7.81 to 125 mg/mL. TLC-bioautography was employed to identify active components, which were primarily located within the mid-polar Rf range. Topical gels were developed using 4% and 5% w/w of the most active extract showing the strongest antibacterial effect, which was found in the Huai Kha Khang cultivar. The 5% gel exhibited inhibition zones of 6–20 mm, stayed stable and maintained its antimicrobial activity after four weeks of storage at 4 ± 1 °C, 25 ± 2 °C, and 45 ± 2 °C. These findings highlight the therapeutic potential of underutilized vetiver leaves as a plant-based resource for anti-acne gel formulations in AMR-sensitive applications.
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痤疮相关细菌中日益严重的抗菌耐药性问题持续削弱标准治疗的有效性。本研究考察了[某植物]叶生物量的抗菌潜力。这些叶子常被丢弃或在当地用作护根以帮助保持土壤湿度，是一种未得到充分利用的农业副产品。使用不同的乙醇和水基溶剂体系从五个品种中提取叶提取物，并针对[痤疮丙酸杆菌、金黄色葡萄球菌、表皮葡萄球菌]进行筛选。50%乙醇提取物显示出最强且最一致的抑制模式，最低抑菌浓度值范围为7.81至125毫克/毫升。采用薄层色谱-生物自显影法鉴定活性成分，这些成分主要位于中极性比移值范围内。使用最具活性的提取物（在怀卡康品种中发现，其显示出最强的抗菌效果）以4%和5%（w/w）制成局部凝胶。5%的凝胶显示出6至20毫米的抑菌圈，在4±1℃、25±2℃和45±2℃储存四周后保持稳定并维持其抗菌活性。这些发现突出了未得到充分利用的香根草叶作为一种植物资源在抗菌耐药性敏感应用中用于抗痤疮凝胶制剂的治疗潜力。
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