Wang Qingdong, Kuang Shanshan, Wang Chunyue, Cheng Panjie, Ma Sitong, Tang Baohong
School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China.
Henan Key Laboratory of Bioactive Macromolecules, Zhengzhou, 450001, China.
Int Microbiol. 2025 Sep 16. doi: 10.1007/s10123-025-00717-9.
Selenium, an essential micronutrient that cannot be endogenously synthesized by mammals, requires exogenous dietary supplementation. Lactic acid bacteria can biotransform sodium selenite (Na₂SeO₃) into bioavailable selenium nanoparticles (SeNPs), creating multifunctional selenium-enriched forages. This study systematically assessed the synergy of SeNPs and Pediococcus acidilactici in alfalfa silage through four treatments: control (CK), P. acidilactici alone (LP), Na₂SeO₃ alone (Se), and combined treatment (LPSe). After 30 days of ensiling, fermentation quality, microbial community, and aerobic stability were determined. The results revealed that LPSe silage exhibited decreased pH, butyric acid, and binding protein contents compared with CK, while its organic selenium content (1.64 mg/kg DM) was Significantly higher than that of LP Silage. 16S rRNA sequencing showed increased abundance of Pediococcus and Lactobacillus in LPSe Silage with Simplified bacterial community structure. After 7 days of aerobic exposure, CK Silage showed rapid pH and ammonia Nitrogen increase, whereas LPSe Silage had 28.24% longer aerobic stability than CK and 11.50% longer than LP. These results demonstrate LPSe as the optimal treatment for alfalfa silage.
硒是一种必需的微量营养素，哺乳动物无法内源性合成，需要通过外源膳食补充。乳酸菌可将亚硒酸钠（Na₂SeO₃）生物转化为生物可利用的硒纳米颗粒（SeNPs），从而制备出多功能富硒饲料。本研究通过四种处理方式系统评估了SeNPs与嗜酸乳杆菌在苜蓿青贮中的协同作用：对照（CK）、单独添加嗜酸乳杆菌（LP）、单独添加亚硒酸钠（Se）以及联合处理（LPSe）。青贮30天后，测定发酵品质、微生物群落及有氧稳定性。结果显示，与CK相比，LPSe青贮的pH值、丁酸含量和结合蛋白含量均降低，而其有机硒含量（1.64 mg/kg干物质）显著高于LP青贮。16S rRNA测序表明，LPSe青贮中嗜酸乳杆菌属和乳杆菌属的丰度增加，细菌群落结构简化。有氧暴露7天后，CK青贮的pH值和氨态氮迅速增加，而LPSe青贮的有氧稳定性比CK长28.24%，比LP长11.50%。这些结果表明LPSe是苜蓿青贮的最佳处理方式。
