住院COVID-19患者中的Toll样受体失调

Toll-Like Receptor Dysregulation in The Hospitalized COVID-19 Patients.

作者信息

Kamiab Zahra, Kazemi Arababadi Mohammad, Bahrehmand Fatemeh, Bazmandegan Gholamreza, Sayadi Ahmadreza, Abbasifard Mitra

机构信息

Department of Community Medicine, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

Department of Laboratory Sciences, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

出版信息

Adv Biomed Res. 2025 Aug 26;14:78. doi: 10.4103/abr.abr_287_22. eCollection 2025.

DOI:10.4103/abr.abr_287_22

PMID:40958920

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12435631/

Abstract

BACKGROUND

A severe pro-inflammatory feedback is the main reason for novel coronavirus (COVID-19)-related complications. Here we intended to investigate the potential involvement of toll-like receptors (TLRs)3, TLR7, TLR8, and TLR9 in the etiopathogenesis of COVID-19.

MATERIALS AND METHODS

mRNA expression of TLR3, TLR7, TLR8, and TLR9 were evaluated in blood samples from 30 COVID-19-infected patients and 30 healthy controls by means of real-time polymerase chain reaction (PCR) approach.

RESULTS

The mRNA expressions of TLR3 ( = 0.038) and TLR9 ( = 0.009) significantly increased in patients with COVID-19 compared with healthy controls. Additionally, the mRNA expression of TLR3 was significantly higher in the male than in female COVID-19 patients ( = 0.020). Experiments indicated that the mRNA expression of TLRs was not significantly different between symptomatic and non-symptomatic COVID-19 subjects. Furthermore, no correlation was detected between mRNA expression of TLRs and patient's clinicopathological data.

CONCLUSION

It seems that TLR3 and TLR9 are involved during COVID-19 infection and might take part in the inflammatory outcome of the patients.

摘要

背景

严重的促炎反馈是新型冠状病毒（COVID-19）相关并发症的主要原因。在此，我们旨在研究Toll样受体（TLR）3、TLR7、TLR8和TLR9在COVID-19发病机制中的潜在作用。

材料与方法

采用实时聚合酶链反应（PCR）方法，对30例COVID-19感染患者和30例健康对照者的血液样本中TLR3、TLR7、TLR8和TLR9的mRNA表达进行评估。

结果

与健康对照相比，COVID-19患者中TLR3（P = 0.038）和TLR9（P = 0.009）的mRNA表达显著增加。此外，男性COVID-19患者中TLR3的mRNA表达显著高于女性（P = 0.020）。实验表明，有症状和无症状的COVID-19患者之间TLR的mRNA表达无显著差异。此外，未检测到TLR的mRNA表达与患者临床病理数据之间存在相关性。