富血小板血浆与纳米姜黄素对弱畸精子症男性冻融精液精子质量参数影响的比较：一项实验室试验研究。

Comparison of the effects of platelet-rich plasma and nanocurcumin on the sperm quality parameters in frozen-thawed semen of men with asthenoteratozoospermia: A lab trial study.

作者信息

Soltani Mahsa, Shojafar Elham, Ghafarizadeh Ali Asghar, Moslemi Azam, Mashayekhi Farideh Jalali, Baazm Maryam

机构信息

Students Research Committee, Arak University of Medical Sciences, Arak, Iran.

Rastak Infertility Treatment Center, Sina Hospital, Arak, Iran.

出版信息

Int J Reprod Biomed. 2025 Aug 27;23(7):533-544. doi: 10.18502/ijrm.v23i7.19481. eCollection 2025 Jul.

DOI:10.18502/ijrm.v23i7.19481

PMID:40959131

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12435332/

Abstract

BACKGROUND

Cryopreservation of sperm can adversely affect sperm quality, particularly in individuals with asthenoteratozoospermia. Various substances are added to the cryopreservation medium to prevent cellular damage.

OBJECTIVE

This study aimed to evaluate the effects of platelet-rich plasma (PRP) and nanocurcumin (nCur) on sperm parameters, DNA fragmentation, and oxidative stress levels in cryopreserved semen.

MATERIALS AND METHODS

In this lab trial study, semen samples of 20 men with asthenoteratozoospermia who referred to Rastak and Ghavamzadeh Infertility Centers, Arak, Iran from June-August 2024 were collected. Each sample was divided into 5 groups: control (no treatment), PRP, PRP, nCur, and nCur. The control samples underwent freezing without additives. Post-thawing assessments included sperm motility, viability, DNA fragmentation index, and malondialdehyde levels to evaluate oxidative stress.

RESULTS

Treatment with PRP (p 0.001) and nCur (p = 0.001) significantly increased sperm motility compared to the control group. All treatment groups showed a significant increase in viable sperm following freezing (p 0.001), with PRP demonstrating the most pronounced effects (p 0.001). While no improvements in sperm morphology were observed after freezing, PRP and nCur significantly reduced DNA fragmentation index after freezing (p 0.001), with PRP being the most effective (p 0.001). Additionally, both PRP and nCur significantly decreased malondialdehyde levels compared to the control group (p 0.001).

CONCLUSION

PRP and nCur effectively improve sperm parameters in cryopreserved semen from individuals with asthenoteratozoospermia, particularly emphasizing the superior efficacy of PRP at a concentration of 50 in improving sperm quality. These results support the potential use of these agents as additives in sperm cryopreservation protocols to improve reproductive outcomes.

摘要

背景

精子冷冻保存会对精子质量产生不利影响，尤其是在弱畸精子症患者中。为防止细胞损伤，会在冷冻保存介质中添加各种物质。

目的

本研究旨在评估富血小板血浆（PRP）和纳米姜黄素（nCur）对冷冻保存精液中精子参数、DNA片段化及氧化应激水平的影响。

材料与方法

在这项实验室试验研究中，收集了2024年6月至8月转诊至伊朗阿拉克的Rastak和Ghavamzadeh不孕不育中心的20名弱畸精子症男性的精液样本。每个样本分为5组：对照组（不治疗）、PRP组、PRP组、nCur组和nCur组。对照样本在无添加剂的情况下进行冷冻。解冻后评估包括精子活力、存活率、DNA片段化指数和丙二醛水平，以评估氧化应激。

结果

与对照组相比，PRP（p < 0.001）和nCur（p = 0.001）处理显著提高了精子活力。所有处理组在冷冻后活精子数量均显著增加（p < 0.001），其中PRP组效果最为显著（p < 0.001）。冷冻后精子形态未见改善，但PRP和nCur显著降低了冷冻后的DNA片段化指数（p < 0.001），PRP组最为有效（p < 0.001）。此外，与对照组相比，PRP和nCur均显著降低了丙二醛水平（p < 0.001）。