精子鱼精蛋白1和鱼精蛋白2转录本含量与父亲年龄的关联：一项横断面研究。

Association of sperm protamine1 and protamine2 transcript content with paternal age: A cross-sectional study.

作者信息

Dolatshahi Baharan, Dorostghoal Mehran, Galehdari Hamid, Hemadi Masoud

机构信息

Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Biotechnology and Bioscience Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

出版信息

Int J Reprod Biomed. 2025 Aug 20;23(6):507-516. doi: 10.18502/ijrm.v23i6.19404. eCollection 2025 Jun.

DOI:10.18502/ijrm.v23i6.19404

PMID:40959470

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12435331/

Abstract

BACKGROUND

Over the last decade, there has been a significant increase in average paternal age.

OBJECTIVE

The present study investigated the effects of male age on sperm parameters, DNA fragmentation, andprotamine1 () and protamine2() transcript content in normozoospermic men.

MATERIALS AND METHODS

In this cross-sectional study, 106 semen samples from normozoospermic men were obtained. The objects were divided into 3 age groups: 20-25, 30-35, and 40-45 yr. Sperm parameters and DNA fragmentation were assessed, and transcript levels of and were analyzed in ejaculated spermatozoa.

RESULTS

The highest levels of sperm concentration, motility, and normal morphology were observed in men aged between 20 and 25 yr. Significant declines were seen in sperm total motility (p = 0.006) and normal morphology (p = 0.015) after 40 yr compared to younger men. Significantly (p 0.001) higher levels of DNA damage were seen in 40-45-yr-old men. DNA fragmentation correlated significantly with sperm total motility (r = -0.242, p = 0.012) and normal morphology (r = -0.257, p = 0.008). The lowest levels of and transcripts were seen in 40-45-yr-old men. DNA damage was significantly associated with reduced transcript levelsof (r = -0.453, p = 0.018) and (r = -0.492, p = 0.009). Transcript levels of and in ejaculated spermatozoa were correlated significantly with the age of men.

CONCLUSION

Our findings demonstrate age-related changes in sperm and transcript content and their correlations with sperm parameters and DNA fragmentation.

摘要

背景

在过去十年中，父亲的平均年龄显著增加。

目的

本研究调查了男性年龄对正常精子男性的精子参数、DNA碎片化以及鱼精蛋白1（PRM1）和鱼精蛋白2（PRM2）转录本含量的影响。

材料与方法

在这项横断面研究中，获取了106例正常精子男性的精液样本。研究对象被分为3个年龄组：20 - 25岁、30 - 35岁和40 - 45岁。评估精子参数和DNA碎片化，并分析射出精子中PRM1和PRM2的转录水平。

结果

20至25岁男性的精子浓度、活力和正常形态水平最高。与年轻男性相比，40岁以后精子的总活力（p = 0.006）和正常形态（p = 0.015）显著下降。40 - 45岁男性的DNA损伤水平显著更高（p < 0.001）。DNA碎片化与精子总活力（r = -0.242，p = 0.012）和正常形态（r = -0.257，p = 0.008）显著相关。40 - 45岁男性的PRM1和PRM2转录本水平最低。DNA损伤与PRM1（r = -0.453，p = 0.018）和PRM2（r = -0.492，p = 0.009）转录水平降低显著相关。射出精子中PRM1和PRM2的转录水平与男性年龄显著相关。