Chen Liang-Wei, Wu Kenneth Lap-Kei, Tam Kin-Wai, Ma Chun-Wai, Tsui Yat-Ping, Lai Chun-Hong, Chan Ying-Shing, Shum Daisy Kwok-Yan
Institute of Neurosciences, The Fourth Military Medical University, Xi'an, China.
School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China.
IBRO Neurosci Rep. 2025 Apr 14;18:690-697. doi: 10.1016/j.ibneur.2025.04.008. eCollection 2025 Jun.
Following unilateral loss of vestibular input, recovery of motor symptoms is achieved within 2 weeks in rodents. Given that neurogenesis was only reported at 1 month post-lesion, whether there is neurogenesis in this early phase of vestibular compensation remains to be investigated. If not, what then is the major cell type that participates in this timeframe? We show abundant nestin-positive cells in the ipsilesional but not contralesional vestibular nucleus (VN) of rats after ablating cell bodies of vestibular nerve at the Scarpa's ganglion, as confirmed by both magnetic resonance imaging after surgery and histology. Bromodeoxyuridine (BrdU) uptake indicated that these cells actively proliferated. A high proportion of the cells were double-positive for nestin and GFAP as early as 4 days, and up to 2 weeks post-lesion, in contrast to none in control preparations. In contrast, the number of NeuN-positive neural lineage cells in the VN remained constant in both the control and lesioned rats. Furthermore, NeuN-positive cells were not positive for BrdU. However, a small number of proliferating cells stained positive for the immature neuron progenitor marker doublecortin. Taken together, we show that unilateral loss of vestibular input stimulates proliferation of neuroglial progenitors, and provide evidence that argues against occurrence of neurogenesis within the 2 week period in which recovery of postural and motor symptoms occurs.
在啮齿动物中，单侧前庭输入丧失后，运动症状可在2周内恢复。鉴于神经发生仅在损伤后1个月才被报道，在前庭代偿的这个早期阶段是否存在神经发生仍有待研究。如果不存在，那么在这个时间段内参与的主要细胞类型是什么呢？我们发现，在切除斯卡帕神经节处的前庭神经细胞体后，大鼠患侧而非对侧前庭核（VN）中有大量巢蛋白阳性细胞，术后磁共振成像和组织学均证实了这一点。溴脱氧尿苷（BrdU）摄取表明这些细胞在积极增殖。早在损伤后4天，高达2周时，高比例的细胞巢蛋白和胶质纤维酸性蛋白（GFAP）呈双阳性，而对照制剂中则没有。相比之下，VN中NeuN阳性神经谱系细胞的数量在对照大鼠和损伤大鼠中均保持恒定。此外，NeuN阳性细胞BrdU染色为阴性。然而，少量增殖细胞对未成熟神经元祖细胞标志物双皮质素染色呈阳性。综上所述，我们表明单侧前庭输入丧失刺激了神经胶质祖细胞的增殖，并提供了证据反驳在姿势和运动症状恢复的2周内发生神经发生的观点。
