Li Zhonghao, Cai Zhihan, Wang Yuhan, Liu Yuliang, Li Guifeng, Yang Xi, Deng Ming, Chen Yu-Cheng, Yang Jichun, Luo Yang, Gong Chaoyang, Zhu Tao
Key Laboratory of Optoelectronic Technology and Systems (Ministry of Education of China), School of Optoelectronic Engineering, Chongqing University, Chongqing, 400044, China.
Department of Laboratory Medicine, Chongqing Center for Clinical Laboratory, Chongqing Academy of Medical Sciences, Chongqing General Hospital, School of Medicine, Chongqing University, Chongqing, 401147, China.
Light Sci Appl. 2025 Sep 17;14(1):327. doi: 10.1038/s41377-025-02015-5.
The mechanical properties of biological fluids serve as early indicators of disease, offering valuable insights into complex physiological and pathological processes. However, the existing technologies struggle to achieve high-throughput measurement, limiting their widespread applications in disease diagnosis. Here, we propose laser-emission vibrational microscopy of microdroplets for high-throughput measurement of the intrinsic mechanical properties of fluids. The microdroplet array supporting high Q-factor (10) whispering gallery modes (WGM) lasing was massively fabricated on a superhydrophobic surface with inkjet printing. Ultrasound was employed to actuate the mechanical vibrations of the microdroplets, and the vibration amplitude was quantified using time-resolved laser spectra. We found that the stimulus-response of the laser emission is strongly dependent on the liquid viscosity. Fast mapping of the microdroplets' viscosities was achieved by stage scanning. High-throughput screening of hyperlipidemia disease was also demonstrated by performing over 2000 measurements within 25 min. Thanks to the small volume of the microdroplets, a single drop of blood can support over seven million measurements. The high-throughput ability and small sample consumption make it a promising tool for clinical diagnoses based on mechanical properties.
生物流体的力学性能可作为疾病的早期指标，为复杂的生理和病理过程提供有价值的见解。然而，现有技术难以实现高通量测量，限制了它们在疾病诊断中的广泛应用。在此，我们提出用于高通量测量流体固有力学性能的微滴激光发射振动显微镜。通过喷墨打印在超疏水表面大规模制备了支持高Q因子（10）回音壁模式（WGM）激光的微滴阵列。利用超声波激发微滴的机械振动，并使用时间分辨激光光谱对振动幅度进行量化。我们发现激光发射的刺激响应强烈依赖于液体粘度。通过台架扫描实现了微滴粘度的快速映射。通过在25分钟内进行超过2000次测量，还展示了对高脂血症疾病的高通量筛查。由于微滴体积小，一滴血可支持超过七百万次测量。高通量能力和小样本消耗使其成为基于力学性能的临床诊断的有前途的工具。
