柠檬油的光毒性研究。

A study of the phototoxicity of lemon oil.

作者信息

Naganuma M, Hirose S, Nakayama Y, Nakajima K, Someya T

出版信息

Arch Dermatol Res. 1985;278(1):31-6. doi: 10.1007/BF00412492.

Abstract

Lemon oil contains furocoumarin derivatives and is known to cause phototoxicity. In this study, lemon oil was fractionated, and its phototoxic activity was measured by means of a biological assay. The substances producing phototoxicity were identified by high-performance liquid chromatography as being oxypeucedanin and bergapten. The phototoxic potency of oxypeucedanin was only one-quarter of that of bergapten. However, the amounts of these two phototoxic compounds present in lemon oils produced in different regions of the world varied by a factor of more than 20 (bergapten, 4-87 ppm; oxypeucedanin, 26-728 ppm), and their ratio was not constant. The two compounds accounted for essentially all of the phototoxic activity of all lemon-oil samples. Among various other citrus-essential oils investigated, lime oil and bitter-orange oil also contained large amounts of oxypeucedanin. Oxypeucedanin was found to elicit photopigmentation on colored-guinea-pig skin without preceding visible erythema.

摘要

柠檬油含有呋喃香豆素衍生物，已知会引起光毒性。在本研究中，对柠檬油进行了分馏，并通过生物测定法测量其光毒性活性。通过高效液相色谱法鉴定出产生光毒性的物质为氧化补骨脂素和佛手柑内酯。氧化补骨脂素的光毒性效力仅为佛手柑内酯的四分之一。然而，世界不同地区生产的柠檬油中这两种光毒性化合物的含量相差超过20倍（佛手柑内酯，4 - 87 ppm；氧化补骨脂素，26 - 728 ppm），且它们的比例并不恒定。这两种化合物基本上构成了所有柠檬油样品的全部光毒性活性。在研究的各种其他柑橘属精油中，酸橙油和苦橙油也含有大量的氧化补骨脂素。发现氧化补骨脂素会在有色豚鼠皮肤上引起色素沉着，且之前没有可见的红斑。