Zeromski J
Clin Exp Immunol. 1970 Apr;6(4):633-7.
An antibody against neurones, which has previously been demonstrated by immunofluorescence, is present in the sera of patients with sensory carcinomatous neuropathy. This antibody was demonstrated by a new technique, namely the use of purified peroxidase-labelled sheep anti-human γ-globulin. In cryostat sections of brain treated with a first layer of serum antibody followed by a second layer of peroxidase labelled anti-human γ-globulin, heavy coarse brown deposits in nerve cells only from different parts of the central nervous system indicated a positive reaction. The special technical precautions necessary to obtain specific staining of neurones are outlined.
一种先前已通过免疫荧光法证实的抗神经元抗体存在于感觉性癌性神经病变患者的血清中。这种抗体通过一种新技术得以证实，即使用纯化的过氧化物酶标记的羊抗人γ球蛋白。在用第一层血清抗体处理，接着用第二层过氧化物酶标记的抗人γ球蛋白处理的脑冰冻切片中，仅来自中枢神经系统不同部位的神经细胞中出现粗大的棕色沉积物，表明反应呈阳性。文中概述了获得神经元特异性染色所需的特殊技术预防措施。
