Immunological studies in ulcerative colitis. VI. Light microscopic studies with peroxidase conjugated antibodies.

Cryostat sections of colon from germ-free rats were incubated with sera from patients with ulcerative colitis or healthy controls. With peroxidase-conjugated antibody from sheep to human immunoglobulin, a distinct staining of goblet cell surface was observed with the patients' sera but not with the controls. The staining compares well with that obtained by indirect immunofluorescence. It could easily be distinguished from staining due to endogenous peroxidase.