Zhang Yanyan, Wang Wanning, Piao Chenggui, Li Wenjin, Cotty Peter J, Shan Shihua, Rasheed Usman, Migheli Quirico, Kong Qing
School of Food Science and Engineering, Ocean University of China, Qingdao 266404, China.
Tai'an Academy of Agricultural Sciences, Tai'an 271018, China.
J Fungi (Basel). 2025 Jun 27;11(7):491. doi: 10.3390/jof11070491.
can produce aflatoxins, posing a threat of contamination to peanuts. To mitigate this issue, the use of biocontrol isolates, which do not produce aflatoxins (AF), has been considered to reduce aflatoxin levels. In this study, we evaluated five different AF isolates belonging to different vegetative compatibility groups, all of which exhibited varying degrees of deletion in aflatoxin biosynthesis gene clusters. One isolate that exhibited poor competitive ability against toxigenic was eliminated, and the remaining four isolates were formulated as biocontrol agents and applied to a peanut field in Tai'an, Shandong, as a combination. Three months after application, the soil aflatoxin content was reduced from 0.62 ± 0.01 to 0.19 ± 0.03 μg/kg (inhibition rate: 69.35%). Among filamentous fungi in the soil, the proportion of AF isolates increased from 0% to 4.33%. Using SSR-specific primers, the microbial agents were recovered. We discovered that among the four AF isolates, CA04 had a lower colonization rate compared to the other three (only 12.00% of the total AF population), suggesting that the absence of sclerotia may result in poor reversibility and weaker dispersal ability. We utilized Illumina sequencing to investigate the changes in soil fungal ecology. The results showed a reduction in the population density of harmful fungi, such as spp. (66.18%) and spp. (79.90%), but an increase in the density of . This is the first study on the dispersal distance and soil fungal community structure following the application of AF agents in peanut fields in China.
能产生黄曲霉毒素，对花生构成污染威胁。为缓解这一问题，人们考虑使用不产生黄曲霉毒素（AF）的生物防治分离株来降低黄曲霉毒素水平。在本研究中，我们评估了五个属于不同营养体亲和群的不同AF分离株，它们在黄曲霉毒素生物合成基因簇中均表现出不同程度的缺失。一个对产毒菌株竞争能力较差的分离株被淘汰，其余四个分离株被配制成生物防治剂，并作为组合应用于山东泰安的一个花生田。施用三个月后，土壤黄曲霉毒素含量从0.62±0.01降至0.19±0.03μg/kg（抑制率：69.35%）。在土壤丝状真菌中，AF分离株的比例从0%增加到4.33%。使用SSR特异性引物回收了微生物制剂。我们发现，在四个AF分离株中，CA04的定殖率低于其他三个（仅占总AF群体的12.00%），这表明没有菌核可能导致可逆性差和扩散能力较弱。我们利用Illumina测序研究了土壤真菌生态的变化。结果表明，有害真菌如 spp.（66.18%）和 spp.（79.90%）的种群密度降低，但 的密度增加。这是中国首次关于在花生田施用AF制剂后扩散距离和土壤真菌群落结构的研究。
