无菌豚鼠迟发型超敏反应受损。
Impaired delayed hypersensitivity in germ-free guinea-pigs.
作者信息
Lev M, Battisto J R
出版信息
Immunology. 1970 Jul;19(1):47-54.
The delayed-type hypersensitivity response of germ-free guinea-pigs was found to be defective. Whereas almost all conventionally reared guinea-pigs became hypersensitive to an allergenic hapten (picryl chloride), most germ-free guinea-pigs did not. When injected with a fully antigenic substance, bovine γ-globulin (BGG), none of the germ-free animals acquired BGG-specific delayed hypersensitivity. Further, none of the germ-free guinea-pigs developed spontaneous iso-hypersensitivity for a beta globulin as do conventional guinea-pigs. In addition, germ-free guinea-pigs given Freund's complete adjuvant did not develop the characteristic induration or erythema normally seen at injection sites and most animals died within 21 days. Germ-free guinea-pigs given competent lymphoid cells from highly sensitized conventional guinea-pigs were unable to translate adoptive hypersensitivity into delayed dermal reactions. A permeability factor in aged guinea-pig sera, injected into the skin of germ-free and conventional animals to determine whether the skin of germ-free guinea-pigs was able to support reactions, initiated immediate dermal reactions of equal intensity in both sets of animals.
无菌豚鼠的迟发型超敏反应存在缺陷。几乎所有常规饲养的豚鼠都会对致敏性半抗原（苦味酸氯）产生超敏反应，而大多数无菌豚鼠则不会。当注射完全抗原物质牛γ球蛋白（BGG）时，无菌动物均未获得BGG特异性迟发型超敏反应。此外，无菌豚鼠不会像常规豚鼠那样对β球蛋白产生自发的同种超敏反应。另外，给无菌豚鼠注射弗氏完全佐剂后，注射部位通常不会出现典型的硬结或红斑，大多数动物在21天内死亡。给无菌豚鼠输入高度致敏的常规豚鼠的活性淋巴细胞后，它们无法将过继性超敏反应转化为迟发型皮肤反应。为了确定无菌豚鼠的皮肤是否能够支持反应，将老年豚鼠血清中的一种通透性因子注射到无菌和常规动物的皮肤中，两组动物均引发了强度相同的即刻皮肤反应。
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