Borek F, Battisto J R
Immunology. 1971 Dec;21(6):943-50.
A study has been made of the effect of hapten attached to a non-phagocytosable carrier on the immune responses of guinea-pigs. Fragments of polyurethane sponge covalently linked with picryl groups were found to be non-immunogenic when introduced into the animals by a variety of routes. Animals implanted intraperitoneally with picrylated sponge fragments and subjected to a course of immunization with picryl chloride showed impaired immune responses to the hapten as measured by skin tests and antibody determinations. A critical factor determining whether unresponsiveness resulted was whether the fragment was wrapped in omentum. When the haptenated sponge remained outside the omentum both circulating antibody and delayed dermal reactivity were suppressed. When it was placed within the omentum the suppressive effect was seen on skin sensitivity but not on antibody production. This evidence together with information gathered on cellular infiltrates found in sponge sections suggest that hapten coupled sponge fragments induce immune unresponsiveness by selectively trapping white cells essential at the antigen-recognition stage of the immune response.
一项关于附着在不可吞噬载体上的半抗原对豚鼠免疫反应影响的研究已经展开。通过多种途径将与苦味酰基团共价连接的聚氨酯海绵碎片引入动物体内时，发现其无免疫原性。腹腔内植入苦味酰化海绵碎片并接受苦味酰氯免疫疗程的动物，通过皮肤试验和抗体测定，对该半抗原的免疫反应受损。决定是否产生无反应性的一个关键因素是碎片是否被大网膜包裹。当半抗原化海绵留在大网膜外时，循环抗体和迟发性皮肤反应性均受到抑制。当它置于大网膜内时，对皮肤敏感性有抑制作用，但对抗体产生无抑制作用。这一证据以及在海绵切片中发现的细胞浸润信息表明，半抗原偶联的海绵碎片通过选择性捕获免疫反应抗原识别阶段必需的白细胞来诱导免疫无反应性。
