Goodwin J S, Messner R P, Bankhurst A D, Peake G T, Saiki J H, Williams R C
N Engl J Med. 1977 Nov 3;297(18):963-8. doi: 10.1056/NEJM197711032971802.
We examined the role of a prostaglandin-producing suppressor cell in the hyporesponsiveness to phytohemagglutinin seen in Hodgkin's disease. Addition of indomethacin to phytohemagglutinin cultures of lymphocytes from six patients with Hodgkin's disease resulted in an increase of 182 +/- 60 per cent in 3H-thymidine incorporation versus a 44 +/- 18% increase in 29 controls (mean +/- S.D., P less than 0.001). Without indomethacin the mean response of the lymphocytes in Hodgkin's disease was 48% of that of control. With indomethacin it was 94% of the control value. Phytohemagglutinin cultures of Hodgkin-disease lymphocytes produced approximately fourfold more prostaglandin E2 after 48 hours than did normal lymphocytes (P less than 0.02). Removal of glass-adherent cells markedly decreased the enhancement seen with indomethacin; it reduced prostaglandin E2 production by more than 80% and eliminated the differences in response to phytohemagglutinin between Hodgkin-disease and normal lymphocytes. Thus, a glass-adherent, prostaglandin-producing suppressor cell is responsible for the hyporesponsiveness to phytohemagglutinin seen with Hodgkin-disease lymphocytes.
我们研究了产生前列腺素的抑制细胞在霍奇金病中对植物血凝素反应低下所起的作用。在来自6例霍奇金病患者的淋巴细胞的植物血凝素培养物中加入吲哚美辛后，与29例对照者44±18%的增加相比，3H-胸腺嘧啶核苷掺入量增加了182±60%（均值±标准差，P<0.001）。在没有吲哚美辛的情况下，霍奇金病患者淋巴细胞的平均反应是对照者的48%。使用吲哚美辛后，该值为对照值的94%。霍奇金病淋巴细胞的植物血凝素培养物在48小时后产生的前列腺素E2比正常淋巴细胞多约四倍（P<0.02）。去除玻璃黏附细胞显著降低了吲哚美辛所产生的增强作用；它使前列腺素E2的产生减少了80%以上，并消除了霍奇金病淋巴细胞与正常淋巴细胞对植物血凝素反应的差异。因此，一种玻璃黏附、产生前列腺素的抑制细胞是霍奇金病淋巴细胞对植物血凝素反应低下的原因。
