Maruyama Marie, Nagamatsu Takeshi, Kawauchi Satoko, Nishidate Izumi, Sato Shunichi
Department of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Department of Obstetrics and Gynecology, School of Medicine, International University of Health and Welfare, 4-2 Kozunomori, Narita, Chiba, 286-8520, Japan.
Reprod Sci. 2025 Sep 25. doi: 10.1007/s43032-025-01975-8.
Hypoxic-ischemic encephalopathy (HIE), which precipitates during parturition, causes severe neurological sequelae in the neonate. We have previously reported an optical monitoring system based on diffuse reflectance spectroscopy to evaluate brain tissue damage that precedes HIE. This study aimed to evaluate the effects of resveratrol on the suppression of HIE development using this monitoring system. After left carotid artery ligation, neonatal rats were intraperitoneally injected with 20 or 40 mg/kg resveratrol and subjected to hypoxic stress. HIE lesions were assessed after 24 h using triphenyl tetrazolium chloride staining. HIE developed in the non-treated group and the group treated with 20 mg/kg administration. Optical parameter assessment during hypoxic stress revealed that HIE-induced brain damage was characterized by a decrease in scatter amplitude, an increase in total hemoglobin in the cerebral blood flow, and a reduction in tissue oxygen saturation. HIE development was completely suppressed in the 40 mg/kg group, in which the changes in the optical parameters were also suppressed. These findings suggest that resveratrol exerts protective effects against HIE by enhancing oxygen supply to the brain. Resveratrol administration could be a promising approach for preventing neonatal HIE.
缺氧缺血性脑病（HIE）在分娩过程中发生，会导致新生儿出现严重的神经后遗症。我们之前报道了一种基于漫反射光谱的光学监测系统，用于评估HIE之前的脑组织损伤。本研究旨在使用该监测系统评估白藜芦醇对抑制HIE发展的作用。在结扎左颈动脉后，给新生大鼠腹腔注射20或40mg/kg白藜芦醇，并使其遭受缺氧应激。24小时后使用氯化三苯基四氮唑染色评估HIE损伤。未治疗组和给予20mg/kg治疗的组出现了HIE。缺氧应激期间的光学参数评估显示，HIE诱导的脑损伤表现为散射幅度降低、脑血流量中总血红蛋白增加以及组织氧饱和度降低。40mg/kg组中HIE的发展被完全抑制，该组中光学参数的变化也得到了抑制。这些发现表明，白藜芦醇通过增强对大脑的氧气供应对HIE发挥保护作用。给予白藜芦醇可能是预防新生儿HIE的一种有前景的方法。
