Bach M K, Bloch K J, Austen K F
J Exp Med. 1971 Apr 1;133(4):752-71. doi: 10.1084/jem.133.4.752.
The optimum conditions for antigen-induced release of histamine in the rat IgE and IgGa antibody-mediated systems were studied in vitro. The IgE antibody-mediated reaction could be separated into two steps: preparation of target cells with antibody and challenge with antigen. The optimal conditions for these two steps were distinctly different. Release of histamine by IgGa antibody and antigen could not be separated into two steps, and the optimal conditions for the total reaction were identical to those of the antigen challenge step of the IgE antibody-mediated system.
在体外研究了大鼠IgE和IgGa抗体介导系统中抗原诱导组胺释放的最佳条件。IgE抗体介导的反应可分为两个步骤:用抗体制备靶细胞和用抗原激发。这两个步骤的最佳条件明显不同。IgGa抗体和抗原诱导的组胺释放不能分为两个步骤,且总反应的最佳条件与IgE抗体介导系统的抗原激发步骤的条件相同。