Ndungu Luka, Roberts Donald, Long Lewis, Goguet Emilie, Stubner Alex, Beeman Sean, Lewandowski Stephen, Okech Bernard
Division of Occupational and Environmental Health Sciences, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
Division of Global Public Health, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA.
Insects. 2025 Sep 4;16(9):928. doi: 10.3390/insects16090928.
Hydrogen peroxide (HO) occurs in the environment, including in aquatic environments where mosquitoes might lay eggs. However, little is known about the compound's impact on mosquitoes. We conducted an experiment to determine the effect of HO on oviposition behavior and egg hatching using HO concentrations similar to those in natural aquatic environments. Oviposition behavior was evaluated by dual-choice and multi-choice bioassays. Gravid mosquitoes were placed in cages with containers with different HO concentrations (5, 25, 50, and 100 μM). After 72 h, the number of eggs laid was compared between oviposition sites with and without HO. Additionally, egg hatching was assessed under long-period exposure (48 h) and short-period exposure (2, 4, and 6 h and then in deionized water for up to 48 h). Results showed no significant difference in oviposition preference scores in the multi-choice assay (OAI = -0.135 ± 0.06) ( = 0.138), but a significant difference in the dual-choice assay (0.195 ± 0.01) ( = 0.001). Long-period exposure to HO did not significantly affect hatch rates (11.34%) ( = 0.363), but short-period exposure significantly impacted hatch rates (17%) ( = 0.0001), with period of exposure alone playing a significant role ( < 0.0044). Eggs exposed to 100 μM HO for 2 h ( = 0.0070) and 4 h ( = 0.0036) had significantly higher hatch rates compared to the control. This study demonstrates that low concentrations of HO can influence oviposition site characteristics and egg hatch rates. Combined with other environmental factors, HO can shape the reproductive success of , offering potential strategies for mosquito control.
过氧化氢（HO）存在于环境中，包括蚊子可能产卵的水生环境。然而，关于该化合物对蚊子的影响知之甚少。我们进行了一项实验，以确定与天然水生环境中浓度相似的HO对产卵行为和卵孵化的影响。通过双选和多选生物测定法评估产卵行为。将妊娠蚊子放置在装有不同HO浓度（5、25、50和100μM）容器的笼子中。72小时后，比较有HO和无HO的产卵地点之间的产卵数量。此外，在长期暴露（48小时）和短期暴露（2、4和6小时，然后在去离子水中长达48小时）下评估卵孵化情况。结果显示，在多选试验中产卵偏好分数没有显著差异（OAI = -0.135 ± 0.06）（ = 0.138），但在双选试验中有显著差异（0.195 ± 0.01）（ = 0.001）。长期暴露于HO对孵化率没有显著影响（11.34%）（ = 0.363），但短期暴露显著影响孵化率（17%）（ = 0.0001），仅暴露时间就起着重要作用（ < 0.0044）。与对照组相比，暴露于100μM HO 2小时（ = 0.0070）和4小时（ = 0.0036）的卵孵化率显著更高。这项研究表明，低浓度的HO可以影响产卵地点特征和卵孵化率。与其他环境因素相结合，HO可以影响蚊子的繁殖成功率，为蚊子控制提供潜在策略。
