Ielo Ileana, Bauso Luana Vittoria, Laezza Antonio, Campione Paola, Fabiano Luigi, Pastorello Martina, Marino Andreana, Laurita Alessandro, Pepe Antonietta, Bochicchio Brigida, De Luca Giovanna, Messina Grazia Maria Lucia, Calabrese Giovanna
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences (ChiBioFarAm), University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
Department of Basic and Applied Sciences (DISBA), University of Basilicata, Via Ateneo Lucano 10, 85100 Potenza, Italy.
Int J Mol Sci. 2025 Sep 18;26(18):9114. doi: 10.3390/ijms26189114.
Chronic wounds and post-operative complications generate significant biomedical challenges due to impaired tissue regeneration and persistent microbial infections, often aggravated by biofilm formation and antibiotic resistance. To address these issues, this study investigates the development and in vitro evaluation of electrospun polyvinylpyrrolidone (PVP) scaffolds embedded with silver nanoparticles (AgNPs), designed as multifunctional bioactive platforms for wound healing and implant applications. AgNPs were synthesized and uniformly incorporated into the PVP matrix using optimized electrospinning parameters, harnessing their antimicrobial and anti-inflammatory properties alongside the hydrophilicity, biocompatibility, and chemical stability of PVP. Structural and mechanical characterization, including Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM), homogenous nanoparticle dispersion, and favorable mechanical properties, such as Young's modulus. In vitro cytotoxicity assays with fibroblast cell lines demonstrated good biocompatibility, while antibiofilm activity against revealed significant microbial inhibition. Overall, electrospun PVP+AgNPs scaffolds demonstrate strong potential as multifunctional biomaterials for wound healing and implant coating due to their synergistic capacity to support tissue regeneration and inhibit infection. These promising results highlight the need for further in vitro and in vivo investigation to confirm their therapeutic efficacy, biocompatibility, and long-term stability in physiological environments.
慢性伤口和术后并发症因组织再生受损和持续性微生物感染而带来重大的生物医学挑战，生物膜形成和抗生素耐药性往往会加剧这些问题。为了解决这些问题，本研究调查了嵌入银纳米颗粒（AgNP）的电纺聚乙烯吡咯烷酮（PVP）支架的开发及体外评估，该支架被设计为用于伤口愈合和植入应用的多功能生物活性平台。使用优化的电纺参数合成AgNP并将其均匀地掺入PVP基质中，利用其抗菌和抗炎特性以及PVP的亲水性、生物相容性和化学稳定性。进行了结构和力学表征，包括透射电子显微镜（TEM）和原子力显微镜（AFM）、纳米颗粒均匀分散以及良好的力学性能，如杨氏模量。对成纤维细胞系进行的体外细胞毒性试验表明具有良好的生物相容性，而对……的抗生物膜活性显示出显著的微生物抑制作用。总体而言，电纺PVP+AgNP支架由于其支持组织再生和抑制感染的协同能力，显示出作为用于伤口愈合和植入涂层的多功能生物材料的强大潜力。这些有前景的结果凸显了进一步进行体外和体内研究以确认其在生理环境中的治疗效果、生物相容性和长期稳定性的必要性。
