Rueda-Ruzafa Lola, Campos-Ríos Ana, Rivas-Ramírez Paula, Rodríguez-Castañeda Manuela, Herrera-Pérez Salvador, Lamas José Antonio
Department of Functional Biology and Health Sciences, University of Vigo, 36310 Vigo, Spain.
Neuroscience Research Group, Galicia Sur Health Research Institute (IIS Galicia Sur), SERGAS-UVIGO, 36310 Vigo, Spain.
Int J Mol Sci. 2025 Sep 18;26(18):9119. doi: 10.3390/ijms26189119.
Temperature homeostasis is controlled by the vagus nerve. Thermal information is collected by thermoreceptors present in the viscera and driven across sensory neurons of the nodose ganglia (NG), which in turn send it to the hypothalamus. While transient receptor potential channels (TRPs) are traditionally considered for thermal transduction, TREK channels belonging to the two-pore domain K channels family are emerging as thermosensors, but their role in the NG remains understudied. Patch-clamp recordings revealed that increasing the temperature to physiological levels causes a hyperpolarization of the membrane potential followed by a depolarization and, despite physiological temperature increased the firing rate, we have demonstrated that TREK channels might be taking part in the excitability control by counteracting TRPs' effects. In fact, single-channel experiments revealed an increase in TREK channel open probability and a subsequent rise in their activity in NG neurons. All this indicates that TREK channels, mainly TREK1, may be responsible along with TRPs for the maintenance of the membrane potential at physiological temperature in NG neurons.
体温稳态由迷走神经控制。热信息由内脏中的温度感受器收集，并通过结状神经节（NG）的感觉神经元传递，这些神经元再将其发送到下丘脑。虽然传统上认为瞬时受体电位通道（TRP）参与热传导，但属于双孔结构域钾通道家族的TREK通道正逐渐成为热传感器，但其在NG中的作用仍未得到充分研究。膜片钳记录显示，将温度升高到生理水平会导致膜电位超极化，随后发生去极化，尽管生理温度会增加放电频率，但我们已经证明TREK通道可能通过抵消TRP的作用参与兴奋性控制。事实上，单通道实验显示NG神经元中TREK通道的开放概率增加，随后其活性升高。所有这些表明，TREK通道，主要是TREK1，可能与TRP一起负责维持NG神经元在生理温度下的膜电位。
