抗生素的生物转化：化合物的降解并不总是能消除其毒性。
Biotransformation of Antibiotics by : Degradation of Compounds Does Not Always Eliminate Their Toxicity.
作者信息
Ghariani Bouthaina, Zouari-Mechichi Héla, Alessa Abdulrahman H, Alqahtani Hussain, Alsaigh Ahmad A, Mechichi Tahar
机构信息
Laboratory of Biochemistry and Enzyme Engineering of Lipases, National School of Engineers of Sfax, BP 1173, University of Sfax, Sfax 3038, Tunisia.
Institute of Biotechnology of Sfax, BP "1175", University of Sfax, Sfax 3038, Tunisia.
出版信息
Antibiotics (Basel). 2025 Sep 5;14(9):897. doi: 10.3390/antibiotics14090897.
BACKGROUND/OBJECTIVES: Wastewaters containing antibiotics pose risks to human health and soil ecosystems. In this study, the white-rot fungus (a basidiomycete exhibiting high laccase production) was used for the biotransformation of three antibiotics (50 mg L): tetracycline, chloramphenicol, and sulfanilamide.
METHODS
The biotransformation process was investigated in liquid and solid media using high-performance liquid chromatography (HPLC) and the bacterial growth inhibition agar well diffusion method, respectively.
RESULTS
Among the three antibiotics tested, tetracycline showed the highest biotransformation efficiency, achieving a 100% removal rate in the liquid medium and a 100% decrease in the growth inhibition of in the solid medium. Chloramphenicol and sulfanilamide were partially removed (20% and 16%, respectively) after 12 days of treatment in more than one step without the loss of their antibacterial activities. The presence of these antibiotics in the culture medium of enhanced laccase activity, indicating that this ligninolytic enzyme might participate in the biotransformation process.
CONCLUSIONS
Thus, the results reported in this article extend our knowledge of the catalytic potential of and give further perspectives for its application in the biodegradation of antibiotics. To the best of our knowledge, this is the first study wherein was used for the treatment of tetracycline, chloramphenicol, and sulfanilamide.
背景/目的：含有抗生素的废水对人类健康和土壤生态系统构成风险。在本研究中，白腐真菌（一种高产漆酶的担子菌）被用于三种抗生素（50毫克/升）的生物转化，这三种抗生素分别是四环素、氯霉素和磺胺。
方法
分别使用高效液相色谱法（HPLC）和细菌生长抑制琼脂扩散法，在液体和固体培养基中研究生物转化过程。
结果
在所测试的三种抗生素中，四环素显示出最高的生物转化效率，在液体培养基中的去除率达到100%，在固体培养基中对[细菌名称未给出]的生长抑制降低了100%。氯霉素和磺胺在经过多步处理12天后部分被去除（分别为20%和16%），且未丧失其抗菌活性。这些抗生素在[细菌名称未给出]的培养基中的存在增强了漆酶活性，表明这种木质素分解酶可能参与了生物转化过程。
结论
因此，本文报道的结果扩展了我们对白腐真菌催化潜力的认识，并为其在抗生素生物降解中的应用提供了进一步的前景。据我们所知，这是首次使用白腐真菌处理四环素、氯霉素和磺胺的研究。
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