Tan Zewen, Beltrán-Flores Eduardo, Ramos-Meza Gisselle D, Alonso Lucas L, Sarrà Montserrat
Department d'Enginyeria Química, Biològica i Ambiental, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, 08193, Spain; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou, 510650, China.
Department d'Enginyeria Química, Biològica i Ambiental, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, 08193, Spain.
Environ Pollut. 2025 Aug 1;378:126504. doi: 10.1016/j.envpol.2025.126504. Epub 2025 May 22.
Antibiotics have been abused in livestock as veterinary drug and feed additive. Their incomplete metabolization by animals resulted in heavy accumulation in livestock manure, and therefore they can pose a threat to the environment. In this study, the mechanism of three antibiotics (oxytetracycline (OTC), sulfadiazine (SDZ), enrofloxacin (ENR)) removal/biodegradation by Trametes versicolor pellets in air-pulse fluidized-bed reactor was explored, and the effects of wood immobilized T. versicolor on four antibiotics (OTC, SDZ, ENR and chloramphenicol (CAP)) removal in solid cow manures were evaluated. T. versicolor could remove OTC, SDZ, ENR through adsorption and biodegradation, with the removal efficiency at 92 % and 98 % in 21 h and 98 % after 68 h, respectively. The removal kinetics of those three antibiotics fitted well with the first-order kinetic model, with the removal constant k at -0.238 h, -0.102 h and -0.023 h, respectively. T. versicolor could biodegrade those three antibiotics using laccase and cytochrome P450 system with the order SDZ ≈ OTC > ENR. Furthermore, wood immobilized T. versicolor promoted SDZ, OTC, ENR and chloramphenicol (CAP) antibiotic biodegradation in cow manure, especially in high inoculation ratio (wood immobilized T. versicolor: solid cow manures = 1:2). This study revealed the mechanism of simultaneous SDZ, OTC, ENR and CAP antibiotic removal/biodegradation by white-rot fungi T. versicolor even by wood immobilized T. versicolor in solid cow manures, which provide a theoretical basis for future application of biological removal of antibiotics present in wastewater and solid manures.
抗生素作为兽药和饲料添加剂在牲畜养殖中被滥用。它们在动物体内代谢不完全，导致在牲畜粪便中大量积累，因此会对环境构成威胁。本研究探讨了在空气脉冲流化床反应器中，云芝菌球对三种抗生素（土霉素（OTC）、磺胺嘧啶（SDZ）、恩诺沙星（ENR））的去除/生物降解机制，并评估了木固定化云芝对固体牛粪中四种抗生素（OTC、SDZ、ENR和氯霉素（CAP））去除的影响。云芝可通过吸附和生物降解去除OTC、SDZ、ENR，去除效率分别在21小时达到92%、98%，68小时后达到98%。这三种抗生素的去除动力学与一级动力学模型拟合良好，去除常数k分别为-0.238 h、-0.102 h和-0.023 h。云芝可利用漆酶和细胞色素P450系统对这三种抗生素进行生物降解，降解顺序为SDZ≈OTC>ENR。此外，木固定化云芝促进了牛粪中SDZ、OTC、ENR和氯霉素（CAP）的生物降解，尤其是在高接种比例（木固定化云芝：固体牛粪=1:2）时。本研究揭示了白腐菌云芝甚至木固定化云芝在固体牛粪中同时去除/生物降解SDZ、OTC、ENR和CAP抗生素的机制，为未来生物去除废水和固体粪便中抗生素的应用提供了理论依据。
