Yagci Ismail Tugay, Akdemir Ovunc, Eyuboglu Atilla, Sezak Murat, Aydogdu Semih
Department of Orthopedics and Traumatology, Liv Hospital, Istanbul 34145, Turkey.
Department Plastic, Aesthetic and Reconstructive Surgery, Istanbul Aydin University, Istanbul 34295, Turkey.
Life (Basel). 2025 Sep 5;15(9):1405. doi: 10.3390/life15091405.
This study aimed to evaluate the efficacy of carboxymethylcellulose (Seprafilm) for the prevention and treatment of arthrofibrosis in rabbit knees, as well as to investigate its underlying mechanisms of action against fibrosis and adhesion formation. Sixteen male New Zealand white rabbits were randomly divided into two groups: a control group and a treatment group that received Seprafilm following surgically induced arthrofibrosis in the knee joint. Macroscopic and histological assessments were performed to evaluate adhesion, fibrosis, inflammation, and edema. In the control group, macroscopic adhesion was severe in five rabbits (62.5%), moderate in two (25%), and minimal in one (12.5%). No macroscopic adhesion was observed in the Seprafilm group. The mean adhesion score was 2.5 ± 0.75 in the control group versus 0 in the treatment group ( < 0.001). Histologically, five rabbits (62.5%) in the control group showed significant fibrosis, and three (37.5%) showed moderate fibrosis, whereas all rabbits in the Seprafilm group exhibited only minimal fibrosis ( < 0.001). Seprafilm was effective in reducing both macroscopic and histological signs of adhesion and fibrosis in a rabbit arthrofibrosis model. These findings suggest its potential as a preventive and therapeutic agent in managing arthrofibrosis.
本研究旨在评估羧甲基纤维素（Seprafilm）预防和治疗兔膝关节纤维性关节病的疗效，并探讨其抗纤维化和粘连形成的潜在作用机制。16只雄性新西兰白兔被随机分为两组：对照组和在手术诱导膝关节纤维性关节病后接受Seprafilm治疗的治疗组。进行宏观和组织学评估以评价粘连、纤维化、炎症和水肿情况。在对照组中，5只兔子（62.5%）出现严重的宏观粘连，2只（25%）为中度粘连，1只（12.5%）为轻度粘连。Seprafilm组未观察到宏观粘连。对照组的平均粘连评分为2.5±0.75，而治疗组为0（<0.001）。组织学上，对照组5只兔子（62.5%）表现出明显的纤维化，3只（37.5%）表现为中度纤维化，而Seprafilm组所有兔子仅表现出轻度纤维化（<0.001）。在兔纤维性关节病模型中，Seprafilm在减轻粘连和纤维化的宏观及组织学表现方面均有效。这些发现提示其在管理纤维性关节病方面具有作为预防和治疗药物的潜力。
