Bridi Vanessa, do Prado Débora Pereira Gomes, Ferreira Stéfanne Rodrigues Rezende, Pedretti Carolina Pedrosa, Filho Edmar Gonçalves Pereira, Santos Wagner Gouvêa Dos, Rezende Hanstter Hallison Alves
Laboratory of Bacteriology and Micology, Biomedicine Course, Institute of Health Sciences, Jatobá Campus, Federal University of Jataí, Jataí 75801-615, Goiás, Brazil.
Genetics and Molecular Biology Laboratory, Biomedicine Course, Institute of Health Sciences, Jatobá Campus, Federal University of Jataí, Jataí 75801-615, Goiás, Brazil.
Pathogens. 2025 Aug 24;14(9):845. doi: 10.3390/pathogens14090845.
Micro-organisms are abundant in nature and can also be found in hospital settings, causing high rates of infections. This study aimed to identify bacteria isolated from a veterinary hospital, as well as to perform antimicrobial susceptibility testing using the disk diffusion method (Kirby-Bauer), biofilm production tests using 96-well polystyrene microtiter plates and crystal violet dye, and genetic analysis of the operon of isolates. Three collections were made from eleven surfaces and objects in the hospital's non-critical areas (general areas) and critical areas (surgical center), totaling thirty-three samples. A total of 66 different bacterial isolates were obtained, with 77% (51/66) Gram-positive and 23% (29/66) Gram-negative. Resistance profiles were found for multidrug-resistance (MDR), methicillin-resistant (MRSA), methicillin-resistant (MRSE), and other unidentified species of methicillin-resistant coagulase-negative (MRCNS) and extended-spectrum beta-lactamase (ESBL), as well as biofilm production rates of 57% (38/66) of the isolates. Analysis of the operon genes for sp. showed divergence in some samples when compared to the phenotypic test performed. In summary, there is a high presence of micro-organisms with resistance and virulence factors spread throughout the various areas of the veterinary hospital.
微生物在自然界中大量存在，在医院环境中也能发现，会导致高感染率。本研究旨在鉴定从一家兽医医院分离出的细菌，以及使用纸片扩散法（Kirby-Bauer）进行抗菌药敏试验，使用96孔聚苯乙烯微量滴定板和结晶紫染料进行生物膜生成试验，以及对分离株的操纵子进行基因分析。从医院非关键区域（一般区域）和关键区域（手术中心）的11个表面和物体上进行了三次采样，共采集了33个样本。总共获得了66种不同的细菌分离株，其中77%（51/66）为革兰氏阳性菌，23%（29/66）为革兰氏阴性菌。发现了对多重耐药（MDR）、耐甲氧西林金黄色葡萄球菌（MRSA）、耐甲氧西林表皮葡萄球菌（MRSE）以及其他未鉴定的耐甲氧西林凝固酶阴性葡萄球菌（MRCNS）和超广谱β-内酰胺酶（ESBL）的耐药谱，分离株的生物膜生成率为57%（38/66）。与所进行的表型试验相比，对某菌操纵子基因的分析在一些样本中显示出差异。总之，在兽医医院的各个区域都广泛存在具有耐药性和毒力因子的微生物。
