Núñez Andrea, Lapierre Lisette, Escobar Beatriz, Castro Rodrigo
Escuela de Medicina Veterinaria, Facultad de Ciencias Agrarias y Forestales, Universidad Católica del Maule, Curicó, Chile.
Dermatovet Talca, Clínica Veterinaria, Talca, Chile.
Open Vet J. 2025 Jun;15(6):2895-2902. doi: 10.5455/OVJ.2025.v15.i6.59. Epub 2025 Jun 30.
Otitis frequently affects dogs. Underlying diseases and predisposing factors affect the otic environment, thereby causing bacterial proliferation. One of the most isolated species in cases of otitis is the genus, which has widely reported antimicrobial resistance profiles. This has not yet been studied in Talca, Chile.
The objective of this study was to determine the existence of antimicrobial resistance in strains isolated from the external auditory canal of a group of dogs diagnosed with otitis externa in Talca, Chile.
Samples were taken from the external ear canal of 20 dogs with otitis externa from October 2023 to August 2024 at dermatological consultations in the city of Talca. The samples were transported to be processed and analyzed in the MICROVET Veterinary Microbiology laboratory, of the Faculty of Veterinary and Livestock Sciences of the University of Chile, using VITEK® 2 equipment for the identification and determination of antimicrobial susceptibility. The association between the epidemiological variables of the patients and the antimicrobial susceptibility of strains was determined.
Staphylococcus spp. isolation was obtained in 8 samples, 62.5% of which were strains of the coagulase-positive Staphylococcus group, of which 25% were identified as Staphylococcus aureus and 37.5% as Staphylococcus pseudintermedius. The remaining 37.5% were coagulase-negative Staphylococcus strains, which were identified as Staphylococcus chromogenes (12.5%), Staphylococcus simulans (12.5%), and Staphylococcus lentus (12.5%). 50 % of the isolates were resistant, with Staphylococcus pseudintermedius being MDR, with resistance to 6 antibiotics, andS. lentus resistant to 5, both of which were also MRS. There was no association between the epidemiological variables of the patients and the antimicrobial susceptibility of the Staphylococcus strains.
These preliminary results are the first report in Chile demonstrating the presence of methicillin-resistant in a group of dogs with otitis externa in Talca, Chile, South America using VITEK®. These results are relevant from a public health perspective given the close contact between owners, veterinarians, and dogs with otitis externa. These preliminary data contribute to the local understanding of this global phenomenon and provide information to support the theoretical framework for future local studies on epidemiological surveillance.
中耳炎经常影响犬类。潜在疾病和诱发因素会影响耳部环境，从而导致细菌增殖。中耳炎病例中最常分离出的菌种之一是葡萄球菌属，该属具有广泛报道的抗菌药物耐药谱。在智利塔尔卡尚未对此进行研究。
本研究的目的是确定从智利塔尔卡一组被诊断为外耳炎的犬只外耳道分离出的葡萄球菌菌株中是否存在抗菌药物耐药性。
于2023年10月至2024年8月在塔尔卡市的皮肤科会诊中，从20只患有外耳炎的犬只的外耳道采集样本。样本被送往智利大学兽医学院的MICROVET兽医微生物实验室进行处理和分析，使用VITEK® 2设备进行鉴定和确定抗菌药物敏感性。确定了患者的流行病学变量与葡萄球菌菌株的抗菌药物敏感性之间的关联。
在8个样本中分离出葡萄球菌属，其中62.5%是凝固酶阳性葡萄球菌组的菌株，其中25%被鉴定为金黄色葡萄球菌，37.5%被鉴定为中间型葡萄球菌。其余37.5%是凝固酶阴性葡萄球菌菌株，分别被鉴定为产色葡萄球菌（12.5%）、模仿葡萄球菌（12.5%）和缓慢葡萄球菌（12.5%）。50%的分离株具有耐药性，中间型葡萄球菌为多重耐药，对6种抗生素耐药，缓慢葡萄球菌对5种抗生素耐药，二者均为耐甲氧西林菌株。患者的流行病学变量与葡萄球菌菌株的抗菌药物敏感性之间没有关联。
这些初步结果是智利的首份报告，证明在南美洲智利塔尔卡的一组患有外耳炎的犬只中存在耐甲氧西林葡萄球菌，使用的是VITEK®。鉴于主人、兽医与患有外耳炎的犬只之间密切接触，这些结果从公共卫生角度来看具有重要意义。这些初步数据有助于当地对这一全球现象的理解，并为支持未来当地流行病学监测研究的理论框架提供信息。
