de Sousa Raimundo Leoberto Torres, Araujo-Pereira Thais, Vasconcelos Silvia Alcântara, Freire Simone Mousinho, Lima Oriana Bezerra, Dos Santos-Mallet Jacenir Reis, Vilela Mauricío Luiz, Vasconcelos Victor Manoel de Sousa, de Andrade Etielle Barroso, Gomes Régis, Teixeira Clarissa, Carvalho Bruno Moreira, Pita-Pereira Daniela, Britto Constança
Laboratório de Biologia Molecular e Doenças Endêmicas-LABIMDOE, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil.
Laboratório de Vigilância Entomológica-LVE Fundação Oswaldo Cruz, Teresina 64051-110, Brazil.
Pathogens. 2025 Sep 16;14(9):930. doi: 10.3390/pathogens14090930.
Leishmaniasis is difficult to control due to clinical and vector diversity associated with the complex life cycle of parasites, which are transmitted by sandflies. This study investigated the presence of DNA in sandfly vectors, their blood meal sources, and their distribution in relation to environmental and climatic variables in four municipalities in Piauí state, Brazil. Between 2020 and 2022, sandflies were collected, morphologically identified, and analyzed for the presence of parasite DNA and blood meal sources (PCR, sequencing). Climate data were correlated with the density of collected insects. Among the 10,245 specimens collected, (54.87%) and (30.41%) were the most abundant in the collection areas. DNA was detected in , while and DNAs were recovered from was the main blood meal source (~73%). Vector density was associated with humidity, temperature, and precipitation in Teresina and Pedro II, with significant results for . In conclusion, , widely adapted to anthropized environments, can act as a potential vector of the etiological agent of cutaneous leishmaniasis in Teresina and Oeiras. In Pedro II, the detection of DNA in suggests a possible role of this species in the transmission cycle of visceral leishmaniasis, reinforcing the complex ecoepidemiology of spp. in Piauí.
利什曼病难以控制，因为其临床和病媒具有多样性，这与通过白蛉传播的寄生虫复杂生命周期有关。本研究调查了巴西皮奥伊州四个市的白蛉病媒中DNA的存在情况、其血餐来源以及它们相对于环境和气候变量的分布。在2020年至2022年期间，收集了白蛉，进行形态学鉴定，并分析寄生虫DNA和血餐来源（聚合酶链反应、测序）。将气候数据与收集到的昆虫密度进行关联。在所收集的10245个标本中，[具体物种1]（54.87%）和[具体物种2]（30.41%）在收集区域最为丰富。在[具体物种1]中检测到了寄生虫DNA，而从[具体物种2]中回收了[具体寄生虫1]和[具体寄生虫2]的DNA。人类是主要血餐来源（约73%）。在特雷西纳和佩德罗二世，病媒密度与湿度、温度和降水有关，[具体物种]的结果显著。总之，[具体物种]广泛适应人为环境，可作为特雷西纳和奥埃拉斯皮肤利什曼病病原体的潜在病媒。在佩德罗二世，在[具体物种]中检测到[具体寄生虫]的DNA，表明该物种在内脏利什曼病传播循环中可能发挥作用，强化了皮奥伊州[白蛉物种]复杂的生态流行病学。
