Zhou Zikai, Bao Zhuo, Miao Di, Zhou Yuxi, Niu Niu, Wuriyanghan Hada
Key Laboratory of Forage and Endemic Crop Biotechnology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China.
Plants (Basel). 2025 Sep 9;14(18):2820. doi: 10.3390/plants14182820.
Genes involved in disease resistance are crucial for plant immune systems, yet their transcriptional regulatory mechanisms remain poorly understood. , a key member of the soybean mosaic virus resistance cluster (SRC), encodes a Ca-binding EF-hand domain and possesses antiviral activity, but its expression regulation is unclear. Here, we systematically analyzed 4085 soybean () transcriptome datasets and conducted SMV inoculation experiments to characterize expression patterns. -acting element analysis identified 12 regulatory elements in the promoter, including salicylic acid (SA)-responsive elements. Furthermore, a :: reporter vector was constructed and functional analysis was performed in tobacco () and transgenic . exhibited significantly higher basal expression than typical resistance genes ( genes) and was induced by SMV infection, SA treatment, and Ca supplementation, with peak expression at 2-5 h post-treatment (hpi). In transgenic tobacco overexpressing , neither SMV nor Ca could induce :: expression, demonstrating that transcriptional regulation is mediated through SA signaling pathways. showed predominant expression in roots and leaves and responded to temperature stress. Transgenic plants overexpressing exhibited enhanced tolerance to both 12 °C and 37 °C temperature stress. This study elucidates the molecular mechanisms underlying transcriptional regulation through Ca and SA signaling pathways, revealing its dual role in both biotic and abiotic stress responses, especially in temperature stress.
参与抗病性的基因对植物免疫系统至关重要，但其转录调控机制仍知之甚少。GmRRS1，大豆花叶病毒抗性簇（SRC）的关键成员，编码一个钙结合EF手结构域并具有抗病毒活性，但其表达调控尚不清楚。在这里，我们系统地分析了4085个大豆转录组数据集，并进行了大豆花叶病毒接种实验以表征GmRRS1的表达模式。顺式作用元件分析在GmRRS1启动子中鉴定出12个调控元件，包括水杨酸（SA）响应元件。此外，构建了GmRRS1::GUS报告载体并在烟草和转基因大豆中进行了功能分析。GmRRS1表现出比典型抗性基因（R基因）显著更高的基础表达，并受到大豆花叶病毒感染、SA处理和钙补充的诱导，在处理后2 - 5小时（hpi）表达达到峰值。在过表达GmRRS1的转基因烟草中，大豆花叶病毒和钙均不能诱导GmRRS1::GUS表达，表明GmRRS1的转录调控是通过SA信号通路介导的。GmRRS1在根和叶中主要表达并对温度胁迫有响应。过表达GmRRS1的转基因植物对12°C和37°C温度胁迫均表现出增强的耐受性。本研究阐明了通过钙和SA信号通路对GmRRS1转录调控的分子机制，揭示了其在生物和非生物胁迫响应中的双重作用，特别是在温度胁迫方面。
