Huang Kai, Sekine Ken-Taro, Li Shuxin, Xu Hao, Song Daiqiao, Kachroo Pradeep, Kachroo Aardra, Luan Hexiang
Institute of Plant Genetic Engineering, College of Life Sciences, Qingdao Agricultural University, Qingdao, Shandong, 266109, P.R. China.
Department of Plant Pathology, University of Kentucky, Lexington, KY, 40546, USA.
BMC Plant Biol. 2025 Sep 1;25(1):1171. doi: 10.1186/s12870-025-06579-7.
Glycerol 3-phosphate dehydrogenase (G3PDH) mediated the reduction of dihydroxyacetone phosphate to generate Glycerol 3-phosphate (G3P). G3P plays a significant role in plant anti-viral systemic acquired resistance (SAR). Therefore, it is crucial to systematically characterize the G3PDH gene family, especially its role in virus infection in soybean, to facilitate the cultivation of disease-resistant soybean seeds.
In this study, 10 members of the G3PDH family were identified in soybean and renamed according to their chromosomal positions. These members are unevenly distributed across chromosomes. These G3PDH genes were divided into five groups through analysis of conserved motifs, gene structure, and phylogenetic analysis. Findings revealed that anaerobic induction response elements, known to be involved in plant stress resistance, were the most abundant among the identified cis-acting elements. Expression analysis revealed that G3PDH8 exhibited predominant expression in seeds and was significantly upregulated following viral infection. In addition, soybean G3PDH silenced which generated based on bean pod mosaic virus (BPMV) plants accumulated a higher viral load compared to control V plants.
This study provides a systematic characterization of the G3PDH gene family, including protein functional features, expression patterns and bioinformatic analysis. Results laid a foundation for exploring the function of G3PDH gene in soybean antiviral infection and breeding of soybean for SMV resistance.
3-磷酸甘油脱氢酶（G3PDH）介导磷酸二羟丙酮的还原以生成3-磷酸甘油（G3P）。G3P在植物抗病毒系统获得性抗性（SAR）中发挥重要作用。因此，系统地表征G3PDH基因家族，特别是其在大豆病毒感染中的作用，对于促进抗病大豆种子的培育至关重要。
在本研究中，在大豆中鉴定出10个G3PDH家族成员，并根据它们在染色体上的位置重新命名。这些成员在染色体上分布不均。通过对保守基序、基因结构和系统发育分析，将这些G3PDH基因分为五组。研究结果表明，已知参与植物抗逆性的厌氧诱导反应元件在所鉴定的顺式作用元件中最为丰富。表达分析表明，G3PDH8在种子中表现出优势表达，并且在病毒感染后显著上调。此外，与对照V植株相比，基于菜豆荚斑驳病毒（BPMV）产生的大豆G3PDH沉默植株积累了更高的病毒载量。
本研究对G3PDH基因家族进行了系统表征，包括蛋白质功能特征、表达模式和生物信息学分析。研究结果为探索G3PDH基因在大豆抗病毒感染中的功能以及培育抗大豆花叶病毒（SMV）的大豆品种奠定了基础。
