Bai Xue, Yang Caixia, Wang Jing, Zhang Song, Cao Mengji
Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Engineering, Shenyang University, Dadong, Shenyang, Liaoning, 110044, China.
Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, National Citrus Engineering and Technology Research Center, Southwest University, Tiansheng Road, Chongqing, Beibei, 400712, China.
Arch Virol. 2025 Sep 27;170(10):214. doi: 10.1007/s00705-025-06418-y.
A new bipartite idaeovirus tentatively named "Cynanchum rostellatum idaeovirus 1" (CRIV-1) was identified inCynanchum rostellatum exhibiting yellow spots and yellow mosaic symptoms in China. CRIV-1 RNA-1 is 5,418 nucleotides (nt) in length and encodes a polyprotein containing methyltransferase (MTR), helicase (Hel), and RNA-dependent RNA polymerase (RdRp) domains. RNA-2 is 2,224 nt in length and encodes a putative movement protein (MP) and a coat protein (CP). CRIV-1 was found to share the highest nt/amino acid (aa) sequence identity with raspberry bushy dwarf virus (AB948214) in the polyprotein (61.2%/61.8%), with black mulberry idaeovirus (MW017549) in the CP (57.3%/47.4%), and with Actinidia yellowing virus 3 (OL581736) in the MP (59.4%/57.6%). Phylogenetic analysis based on polyprotein aa sequences showed a close relationship of CRIV-1 to other idaeoviruses. CRIV-1 can be transmitted to C. rostellatum andNicotiana benthamiana plants via mechanical inoculation. Collectively, our data support the classification of CRIV-1 as a new member of the genus Idaeovirus.
