Kerek Ádám, Szabó Ábel, Jerzsele Ákos
Department of Pharmacology and Toxicology, University of Veterinary Medicine Budapest, Budapest, Hungary.
National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine Budapest, Budapest, Hungary.
Front Vet Sci. 2025 Sep 12;12:1642902. doi: 10.3389/fvets.2025.1642902. eCollection 2025.
This cross-sectional observational study aimed to determine the antimicrobial susceptibility profiles of isolates from domestic pigeons (Columba livia domestica) in Hungary.
A total of 134 non-redundant isolates were obtained from oropharyngeal and cloacal swabs collected across multiple geographic regions. Antimicrobial susceptibility testing was conducted using the microdilution method, following Clinical and Laboratory Standards Institute (CLSI) guidelines. Minimum inhibitory concentration (MIC) values were determined for a panel of antibiotics relevant to both veterinary and human medicine. Resistance patterns were analyzed using statistical tools including hierarchical clustering, network graph analysis, decision tree modeling, and Monte Carlo simulation.
Multidrug-resistant (MDR) strains constituted 65.7% of the total, while extensive drug-resistant (XDR) and pan-drug-resistant (PDR) strains were found in 4.5% and 1.5% of isolates, respectively. The highest resistance rates were observed for neomycin (76.1%) and florfenicol (72.4%), whereas ceftriaxone and imipenem showed the lowest resistance rates (0.7% and 1.5%). Correlation analysis indicated notable associations between resistance to neomycin, doxycycline, and florfenicol, suggesting potential cross-resistance mechanisms. Monte Carlo simulation estimated an average MDR prevalence of 64.4% (95% CI: 50.0-77.6%). The high prevalence of antimicrobial resistance among pigeon-derived isolates underscores the potential public health risks posed by avian reservoirs within the One Health context.
These findings highlight the need for enhanced AMR surveillance and responsible antibiotic stewardship in veterinary settings. Further molecular investigations are warranted to elucidate the genetic basis of resistance in this population.
这项横断面观察性研究旨在确定匈牙利家鸽（Columba livia domestica）分离株的抗菌药物敏感性谱。
从多个地理区域采集的口咽和泄殖腔拭子中获得了总共134株非重复分离株。按照临床和实验室标准研究所（CLSI）指南，使用微量稀释法进行抗菌药物敏感性测试。确定了一组与兽医学和人类医学相关的抗生素的最低抑菌浓度（MIC）值。使用包括层次聚类、网络图分析、决策树建模和蒙特卡洛模拟在内的统计工具分析耐药模式。
多重耐药（MDR）菌株占总数的65.7%，而广泛耐药（XDR）和泛耐药（PDR）菌株分别在4.5%和1.5%的分离株中发现。新霉素（76.1%）和氟苯尼考（72.4%）的耐药率最高，而头孢曲松和亚胺培南的耐药率最低（0.7%和1.5%）。相关性分析表明新霉素、强力霉素和氟苯尼考耐药之间存在显著关联，提示潜在的交叉耐药机制。蒙特卡洛模拟估计平均MDR患病率为64.4%（95%CI：50.0 - 77.6%）。鸽源分离株中抗菌药物耐药性的高流行率凸显了“同一健康”背景下禽类宿主带来的潜在公共卫生风险。
这些发现强调了在兽医环境中加强抗菌药物耐药性监测和负责任的抗生素管理的必要性。有必要进行进一步的分子研究以阐明该群体耐药性的遗传基础。
