Klein H P, Jahnke L
J Bacteriol. 1971 May;106(2):596-602. doi: 10.1128/jb.106.2.596-602.1971.
In cells of Saccharomyces cerevisiae growing aerobically for 24 hr, acetyl-coenzyme A synthetase [acetate: CoA ligase (AMP), EC 6.2.1.1] was localized principally in the microsomal fraction. On density gradients, the enzyme in such cells behaved as a low-density particle, readily separable from the soluble proteins. After 48 hr of incubation, the cells showed a bimodal distribution of enzyme, with most of the activity now sedimenting with the mitochondrial fraction and only a smaller amount with the microsomal fraction. By using density gradients, two forms of synthetase were obtained from these cells: one band denser and the other band less dense than the intact mitochondria. In all preparations containing synthetase activity, appreciable levels of phospholipids were also detected.
在需氧生长24小时的酿酒酵母细胞中，乙酰辅酶A合成酶[乙酸：CoA连接酶（AMP），EC 6.2.1.1]主要定位于微粒体部分。在密度梯度上，此类细胞中的该酶表现为低密度颗粒，易于与可溶性蛋白质分离。孵育48小时后，细胞呈现出酶的双峰分布，此时大部分活性与线粒体部分一起沉降，只有较少量与微粒体部分一起沉降。通过使用密度梯度，从这些细胞中获得了两种形式的合成酶：一条带比完整线粒体密度大，另一条带比完整线粒体密度小。在所有含有合成酶活性的制剂中，也检测到了相当水平的磷脂。
