Klein H P, Volkmann C, Weibel J
J Bacteriol. 1967 Aug;94(2):475-81. doi: 10.1128/jb.94.2.475-481.1967.
A crude small particle pellet, obtained from postmitochondrial supernatant fractions of Saccharomyces cerevisiae, contains about half the ergosterol and phospholipid of crude cell homogenates. Most of the phospholipid of this pellet is in a "heavy" fraction which, with the aid of electron microscopy, shows membranous elements in addition to discrete particles. The "heavy" fraction, upon treatment with deoxycholate, can be freed of membranes, or, with ribonuclease treatment, ribosomes can be removed, leaving relatively clean membranes. The "heavy" fraction resembles the microsomes of animal cells, but contains considerably less lipids, including phospholipids, thus suggesting a less well-developed intracellular membrane system.
从酿酒酵母线粒体后上清液组分中获得的粗制小颗粒沉淀，其麦角固醇和磷脂含量约为粗制细胞匀浆的一半。该沉淀中的大部分磷脂存在于一个“重”组分中，借助电子显微镜观察，除了离散颗粒外，还可见膜状成分。用脱氧胆酸盐处理该“重”组分可去除膜，或者用核糖核酸酶处理可去除核糖体，从而留下相对纯净的膜。该“重”组分类似于动物细胞的微粒体，但所含脂质（包括磷脂）要少得多，这表明其细胞内膜系统发育程度较低。
