Farrow S P
Br J Exp Pathol. 1977 Aug;58(4):340-51.
The alterations in contents of Na, K and water in thermally injuried explants of guinea-pig flank skin involve changes in both the cells and the interstitial material of the tissue. A full interpretation of the distribution of such changes can only be obtained if the volumes of cellular and extracellular fluid are known. This work examines the techniques of measuring extracellular fluid volume using 14C-inulin and 51Cr EDTA. Although it was found that the inulin preparation was excluded from a considerable part of the extracellular fluid of the skin explants, the space which it defined altered with increasing severity of thermal damage. The relationship of the inulin space with total water and with 51Cr EDTA space, which probably measures the total extracellular volume, provides an insight into the possible importance of changes in the nature of connective tissue in trauma. Alterations in fluid and cation balance between cells and their environment have been discussed in relation to increasing thermal damage. A clear sequence of events following the onset of the injury can be observed.
豚鼠侧腹皮肤热损伤外植体中钠、钾和水含量的变化涉及组织细胞和细胞间质的改变。只有在知道细胞内液和细胞外液体积的情况下，才能对这些变化的分布进行全面解释。这项工作研究了使用14C-菊粉和51Cr乙二胺四乙酸测量细胞外液体积的技术。尽管发现菊粉制剂被排除在皮肤外植体相当一部分细胞外液之外，但它所界定的空间随热损伤严重程度的增加而改变。菊粉空间与总水量以及可能测量总细胞外体积的51Cr乙二胺四乙酸空间之间的关系，有助于深入了解创伤中结缔组织性质变化的潜在重要性。已经讨论了细胞与其环境之间液体和阳离子平衡的变化与热损伤加剧的关系。可以观察到损伤发生后一系列清晰的事件。
