采用离子对萃取、衍生化和气相色谱-电子捕获检测法测定血浆和尿液中的溴甲辛托品。

Determination of oxyphenonium bromide in plasma and urine by means of ion-pair extraction, derivatization and gas chromatography-electron-capture detection.

作者信息

Greving J E, Jonkman J H, Fiks F, de Zeeuw R A, van Bork L E, Orie N G

出版信息

J Chromatogr. 1977 Nov 11;142:611-9. doi: 10.1016/s0021-9673(01)92072-2.

DOI:10.1016/s0021-9673(01)92072-2

Abstract

A sensitive and selective method for the determination of the quaternary ammonium compound oxyphenonium bromide (Antrenyl), a drug with strong anticholinergic properties, in human plasma and urine is described. The method is based on ion-pair extraction of the cation with perchlorate, a re-extraction according to ion-pair principles with tetrapentylammonium as the counter ion, hydrolysis to cyclohexylphenylglycolic acid, derivatization of this acid to its pentafluorobenzyl ester and determination of the ester by gas chromatography and electron-capture detection. Quantitation is possible down to 2 ng/ml of oxyphenonium bromide using 1 ml of plasma and down to 200 ng/ml using 0.1 ml of urine. The method described can also be applied to other anticholinergic drugs with an ester function.

摘要

本文描述了一种灵敏且具选择性的方法，用于测定人血浆和尿液中的季铵化合物溴化氧苯戊铵（安胃灵），该药物具有较强的抗胆碱能特性。该方法基于用高氯酸盐对阳离子进行离子对萃取，以四戊基铵作为反离子按照离子对原理进行再萃取，水解为环己基苯基乙醇酸，将该酸衍生化为其五氟苄酯，并通过气相色谱和电子捕获检测来测定该酯。使用1 ml血浆时，溴化氧苯戊铵的定量下限可达2 ng/ml，使用0.1 ml尿液时可达200 ng/ml。所描述的方法也可应用于其他具有酯功能的抗胆碱能药物。