Wolf K, Darlington R W
J Virol. 1971 Oct;8(4):525-33. doi: 10.1128/JVI.8.4.525-533.1971.
Channel catfish virus was studied in ictalurid fish cell culture, the only system of fish, amphibian, avian, and mammalian cells found to be susceptible. Channel catfish virus infection resulted in intranuclear inclusions and extensive syncytium formation. Replication occurred from 10 to 33 C, but not higher. Best growth was from 25 to 33 C, and the amount of virus released nearly equalled the amount which remained cell-associated. The virus was labile to lipid solvents, and indirect determinations with labeled precursors and a metabolic inhibitor showed evidence of deoxyribonucleic acid. Electron microscopy showed progeny virus, about 100 nm in diameter, in various stages of development in cell nuclei by 4 hr. Present also were nuclear masses of exceptionally electron-dense lamellar material, with a unit dimension of 10 to 15 nm. Virus was enveloped at the nuclear membrane and in cytoplasmic vacuoles, resulting in virions having a diameter of 175 to 200 nm. Negative staining demonstrated icosehedral symmetry and 162 capsomeres. Our data indicate that channel catfish virus is a herpesvirus.
在鲶鱼科鱼类细胞培养中对鲶鱼病毒进行了研究，这是唯一被发现对鱼类、两栖类、鸟类和哺乳类细胞敏感的细胞培养系统。鲶鱼病毒感染导致细胞核内出现包涵体并形成广泛的多核体。病毒在10至33摄氏度之间复制，但温度再高则不能复制。最佳生长温度为25至33摄氏度，释放出的病毒量几乎与仍与细胞结合的病毒量相等。该病毒对脂溶剂不稳定，用标记前体和代谢抑制剂进行的间接测定显示有脱氧核糖核酸的证据。电子显微镜观察显示，到4小时时，细胞核内有直径约100纳米、处于不同发育阶段的子代病毒。还存在单位尺寸为10至15纳米、电子密度极高的层状物质的核团。病毒在核膜和细胞质空泡中被包膜，形成直径为175至200纳米的病毒粒子。负染显示二十面体对称和162个壳粒。我们的数据表明鲶鱼病毒是一种疱疹病毒。
