Dutson T R, Pearson A M, Price J F, Spink G C, Tarrant P J
Appl Microbiol. 1971 Dec;22(6):1152-8. doi: 10.1128/am.22.6.1152-1158.1971.
Myofibrils from pig muscle inoculated and incubated with Pseudomonas fragi showed an extremely disrupted appearance as compared to uninoculated controls. There was an almost complete absence of material in the H zone, marked disruption of the A band (probably myosin), and some loss of dense material from the Z line. These changes indicated that marked proteolysis had occurred. Bacteria observed in spoiled muscle tissue exhibited protrusions or blebs on the outer surface of the cell walls. The blebs appeared to form detached globules that migrated into the muscle mass. Bacteria grown in non-muscle-containing media did not produce blebs, which indicates the blebs were induced by growth on muscle tissue. The possibility that the blebs and globules may contain a proteolytic enzyme responsible for myofibrillar disruption is discussed.
与未接种的对照相比，接种了脆弱拟杆菌并进行培养的猪肌肉肌原纤维呈现出极度紊乱的外观。H 带几乎完全没有物质，A 带（可能是肌球蛋白）明显破坏，Z 线有一些致密物质丢失。这些变化表明发生了明显的蛋白水解。在变质肌肉组织中观察到的细菌在细胞壁外表面呈现出突起或泡状结构。泡状结构似乎形成分离的小球并迁移到肌肉团块中。在不含肌肉的培养基中生长的细菌不会产生泡状结构，这表明泡状结构是由在肌肉组织上生长诱导产生的。文中讨论了泡状结构和小球可能含有负责肌原纤维破坏的蛋白水解酶的可能性。
