Lundgren G, Simmons R L
Clin Exp Immunol. 1971 Dec;9(6):915-26.
Treatment of the stimulatory cells in human one-way mixed lymphocyte cultures with neuraminidase (VCN) caused a marked increase in their stimulatory capacity as measured by an increase in DNA-synthesis by the responding cells. No effect was observed by treating the responding cells with VCN. Heatinactivation of VCN, or incubation in the presence of its specific substrate, N-acetylneuraminlactose, prevented the stimulatory effect. VCN did not increase stimulation in mixed lymphocyte cultures between HL-A identical siblings suggesting that the primary effect is to increase the immunogenicity of HL-A antigens present, probably by facilitating cell-to-cell contact. A low, but statistically significant stimulation occurred in some autologous combinations suggesting that a secondary effect of VCN is to alter, or `unmask', antigenic determinants or mitogenic factors on the stimulatory cells.
用神经氨酸酶（VCN）处理人单向混合淋巴细胞培养物中的刺激细胞，通过反应细胞DNA合成的增加来衡量，其刺激能力显著增强。用VCN处理反应细胞未观察到效果。VCN的热灭活或在其特异性底物N-乙酰神经氨酸乳糖存在下孵育可阻止刺激作用。VCN不会增加HL-A相同同胞之间混合淋巴细胞培养物中的刺激作用，这表明主要作用可能是通过促进细胞间接触来增加存在的HL-A抗原的免疫原性。在一些自体组合中出现了低但具有统计学意义的刺激作用，这表明VCN的次要作用是改变或“暴露”刺激细胞上的抗原决定簇或促有丝分裂因子。
