Biniaminov M, Ramot B, Rosenthal E, Novogrodsky A
Clin Exp Immunol. 1975 Jan;19(1):93-8.
Treatment of human lymphocytes with neuraminidase and galactose oxidase induced extensive blastogenesis. A less pronounced effect was observed after treatment of the cells with galactose oxidase alone. Macrophage-depleted human lymphocytes had a markedly reduced blastogenic response after treatment with neuraminidase and galactose oxidase. Incubation of the purified lymphocytes on macrophage monolayers markedly enhanced their response to neuraminidase and galactose oxidase. Furthermore, a stimulation of thymidine incorporation into purified untreated lymphocytes was noted after incubation of the cells on neuraminidase and galactose oxidase-treated macrophage monolayers.
用神经氨酸酶和半乳糖氧化酶处理人淋巴细胞可诱导广泛的细胞增殖。单独用半乳糖氧化酶处理细胞后，观察到的效应不太明显。用神经氨酸酶和半乳糖氧化酶处理后，巨噬细胞耗竭的人淋巴细胞的细胞增殖反应明显降低。将纯化的淋巴细胞在巨噬细胞单层上孵育，可显著增强它们对神经氨酸酶和半乳糖氧化酶的反应。此外，将细胞在经神经氨酸酶和半乳糖氧化酶处理的巨噬细胞单层上孵育后，发现纯化的未处理淋巴细胞的胸苷掺入受到刺激。
