Caldwell K D, Kesner L F, Myers M N, Giddings J C
Science. 1972 Apr 21;176(4032):296-8. doi: 10.1126/science.176.4032.296.
Protein separation has been achieved by electrical field-flow fractionation, a heretofore unrealized separation technique. Some advantages of this method relative to electrophoresis are the low voltage required, the lack of adverse heating and support effects, and the existence of the method as an elution technique. A comparison of theoretical and experimental retention shows good agreement.
蛋白质分离已通过电场流分级法实现,这是一种此前未实现的分离技术。相对于电泳,该方法的一些优点是所需电压低、不存在不利的加热和支持效应,并且该方法作为一种洗脱技术存在。理论保留值与实验保留值的比较显示出良好的一致性。